Surface plasmon resonance sensor

• US 5,327,225 A
• Filed: 01/28/1993
• Issued: 07/05/1994
• Est. Priority Date: 01/28/1993
• Status: Expired due to Fees

• Current Assignee: Virginia Tech Intellectual Properties, Inc. (Virginia Polytechnic Institute and State University)
• Original Assignee: The Center For Innovative Technology
• Inventors: Dessy, Raymond E., Bender, William J. H.
• Primary Examiner(s): Rosenberger, Richard A.
• Assistant Examiner(s): KEESEE, LACHARLES
• Application Number:
  US08/010,267
• Time in Patent Office: 523 Days
• Field of Search: 356/317, 356/318, 356/445, 250/306, 250/307, 422/82.05, 422/82.09, 422/66, 435/808, 436/515, 436/516
• US Class Current: 356/445
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

An optical fiber surface plasmon resonance (SPR) sensor includes both a metal layer and an overlay or underlay material on its surface. Existing fiber based SPR devices are inherently incapable of monitoring aqueous systems which have a refractive index ranging between 1.33 and 1.35, and existing prism based SPR sensors have proved too cumbersome for online chemical and biochemical analyses. Inclusion of the overlay or underlay material on the SPR sensor allows monitoring media with a refractive index from 1.00 to the 1.39 barrier and above. Hence, the SPR sensor allows monitoring important biochemical and chemical aqueous processes where the media typically have a refractive index between 1.33 and 1.35. In operation, samples are simply applied to the sensing region of the SPR sensor where the metal layer and overlay or underlay materials are coated, introducing a polarized beam of light into the optical fiber, and detecting surface plasmon resonance. The optical fiber can be positioned in a flow cell for on line operations where media are simply moved past the sensing region of the SPR sensor or can be part of a dip stick which is used for batch processes. The SPR sensor has particular utility in monitoring biochemical (antigen-antibody) reactions, detecting corrosion of metal surfaces, and identifying chemical products in an HPLC effluent.

Claims

1. A surface plasmon resonance sensor, comprising:

an optical fiber;

a source of electromagnetic radiation connected to said optical fiber;

first and second films coated on each other and on said optical fiber at a sample contacting region of said optical fiber, said first and second films having a combined thickness small enough to allow an evanescent field portion of a beam of electromagnetic radiation supplied from said source into said optical fiber to penetrate into both said first and second films; and

a detector connected to said optical fiber for detecting surface plasmon resonance as a function of a thickness and refractive index for both said first and second films and a refractive index of said sample.

2. The surface plasmon resonance sensor of claim 1 further comprising a means for conveying said sample to said sample contacting region of said optical fiber.

4. The surface plasmon resonance sensor of claim 1 wherein said first film is selected from the group consisting of silver, gold, chromium, silicon, germanium, and alloys and oxides thereof.

5. The surface plasmon resonance sensor of claim 1, wherein said second film is selected from the group consisting of dielectric materials, polymeric materials, metals, metal oxides, chalcogenides, semiconductors, organic layers, inorganic layers, and glass materials.

6. The surface plasmon resonance sensor of claim 1 wherein said source of electromagnetic radiation is a laser.

7. The surface plasmon resonance sensor of claim 1 further comprising a means to identify changes in surface plasmon resonance.

8. The surface plasmon resonance sensor of claim 1 wherein said optical fiber is a single mode optical fiber.

9. The surface plasmon resonance sensor of claim 1 wherein said first film is a metal or metal alloy and said second film is silicon monoxide.

10. The surface plasmon resonance sensor of claim 1 wherein said optical fiber is less than fifty microns in diameter.

11. The surface plasmon resonance sensor of claim 1 wherein said detector can detect surface plasmon resonance when said sample has a refractive index ranging between 1.00 and 1.39.

12. The surface plasmon resonance sensor of claim 1 wherein said detector can detect surface plasmon resonance when said sample has a refractive index ranging between 1.00 and 1.50.

13. The surface plasmon resonance sensor of claim 1

wherein said first film is selected from the group consisting of silver, gold, chromium, silicon, germanium, and alloys and oxides thereof,

wherein said second film is selected from the group consisting of dielectric materials, polymeric materials, metals, metal oxides, chalcogenides, semiconductors, organic layers, inorganic layers, and glass materials, and

wherein said second film overlies said first film.

14. The surface plasmon sensor of claim 13 wherein said second film is porous.

15. The surface plasmon sensor of claim 13 further comprising a biomaterial specific for binding an analyte of interest in said sample immobilized on said second film.

16. The surface plasmon resonance sensor of claim 1

wherein said first film is selected from the group consisting of silver, gold, chromium, silicon, germanium, and alloys and oxides thereof,

wherein said second film is selected from the group consisting of dielectric materials, polymeric materials, metals, metal oxides, chalcogenides, semiconductors, organic layers, inorganic layers, and glass materials, and

wherein said second film underlies said first film.

17. The surface plasmon sensor of claim 1 wherein said second film comprises two or more materials selected from the group consisting of dielectric materials, polymeric materials, metals, metal oxides, chalcogenides, semiconductors, organic layers, inorganic layers, and glass materials.

3. The surface plasmon resonance sensor of claim 3 wherein said means for conveying includes a flow cell housing said optical fiber.

18. A surface plasmon resonance sensor, comprising:

an optical waveguide;

a source of electromagnetic radiation connected to said optical waveguide;

first and second films coated on each other and on said optical waveguide at a sample contacting region of said optical waveguide, said first and second films having a combined thickness small enough to allow an evanescent field portion of a beam of electromagnetic radiation supplied from said source into said optical waveguide to penetrate into both said first and second films; and

a detector connected to said optical waveguide for detecting surface plasmon resonance as a function of a thickness and refractive index for both said first and second films and a refractive index of said sample.

19. A biochemical or biological sensor, comprising:

an optical fiber;

a source of electromagnetic radiation connected to said optical fiber;

first and second films coated on each other and on said optical fiber at a sample contacting region of said optical fiber, said first and second films having a combined thickness small enough to allow an evanescent field portion of a beam of electromagnetic radiation supplied from said source into said optical fiber to penetrate into both said first and second films, said first film being a metal or metal alloy film, said second film being selected from the group consisting of dielectric materials, polymeric materials, metals, metal oxides, chalcogenides, semiconductors, organic layers, inorganic layers, and glass materials, said first film being coated on said sample contacting region of said optical fiber and said second film being coated on top of said first film;

a biomaterial specific for binding an analyte of interest in a sample immobilized on said second film; and

a detector connected to said optical fiber for detecting changes in surface plasmon resonance which occur when said biomaterial binds said analyte of interest in said sample as a function of a thickness and refractive index for both said first and second films.

20. A corrosion sensor, comprising:

an optical fiber;

a source of electromagnetic radiation connected to said optical fiber;

first and second films coated on each other and on said optical fiber at a sample contacting region of said optical fiber, said first and second films having a combined thickness small enough to allow an evanescent field portion of a beam of electromagnetic radiation supplied from said source into said optical fiber to penetrate into both said first and second films, said first film being a metal or metal alloy;

a detector connected to said optical fiber for detecting changes in surface plasmon resonance when said metal or metal alloy in said first film corrodes due to exposure to corrosive agents in a sample which contacts said first film, said changes in surface plasmon resonance being a function of a thickness and refractive index for both said first and second films.

21. A corrosion sensor as recited in claim 20 wherein said second film is porous and allows said sample to permeate therethrough to contact said first film.

22. A surface plasmon resonance sensor for monitoring effluent from a liquid chromatography or high performance liquid chromotography column, comprising:

an optical fiber;

a source of electromagnetic radiation connected to said optical fiber;

first and second films coated on each other and on said optical fiber at a sample contacting region of said optical fiber, said first and second films having a combined thickness small enough to allow an evanescent field portion of a beam of electromagnetic radiation supplied from said source into said optical fiber to penetrate into both said first and second films;

means to convey effluent from a liquid chromotography or high performance liquid chromotography column over said sample contacting region of said optical fiber; and

a detector connected to said optical fiber for detecting surface plasmon resonance as a function of a thickness and refractive index for both said first and second films and a refractive index of said effluent.