Enzyme-based biosensors for detecting noble gases

• US 5,328,823 A
• Filed: 11/27/1992
• Issued: 07/12/1994
• Est. Priority Date: 11/27/1992
• Status: Expired due to Fees

• Current Assignee: American Air Liquide Incorporated (L'Air Liquide SA)
• Original Assignee: American Air Liquide Incorporated (L'Air Liquide SA)
• Inventors: Boisrobert, Christine E., Spencer, Kevin C.
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): Mohamed, Abdel A.
• Application Number:
  US07/982,500
• Time in Patent Office: 592 Days
• Field of Search: 435/4, 435/288, 435/291, 435/817, 422/83, 422/85, 422/89
• US Class Current: 435/4
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01M 3/20 using special tracer materials, e.g. dye, fluorescent material, radioactive material
• CPC Class Codes: G01N 33/0036 specially adapted to detect a particular component physical analysis of gaseous biological material G01N33/497
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A method of detecting at least one noble gas through a semi-permeable membrane, allowing the permeating noble gas to contact an enzyme, the activity of which is changed by the noble gas, and detecting the change in enzyme activity.

Claims

1. A method of detecting at least one noble gas with an enzyme-based sensor having at least one enzyme therein and having a membrane therefor which is at least semi-permeable to said at least one noble gas, which comprises:

a) permeating said at least one noble gas through said membrane;

b) contacting at least one enzyme in said sensor with an amount of said at least one noble gas sufficient to change an activity of said at least one enzyme; and

c) detecting the change in the activity of said at least one enzyme; and

wherein said at least one noble gas is selected from the group consisting of xenon, krypton, argon and neon.

2. The method of claim 1, wherein said at least one noble gas is in admixture with a carrier gas selected from the group consisting of N₂ O, He, CO₂, N₂, O₂ and air in said mixture of gases.

3. The method of claim 1, wherein said at least one noble gas is at a pressure of from about 10^-2 atmospheres to about 10 atmospheres.

4. The method according to claim 1, wherein said at least one noble gas is in gaseous form or liquid form or both and is injected into contact with said at least one enzyme in said sensor.

5. The method according to claim 1, wherein said at least one enzyme in said sensor is saturated to more than 50 volume % of its full saturation level.

6. The method according to claim 5, wherein said at least one enzyme in said sensor is saturated to more than 70% by volume of its full saturation level.

7. The method according to claim 6, wherein said at least one enzyme in said sensor is saturated to more than 80% by volume of its full saturation level.

8. The method according to claim 2, wherein said admixture contains, in addition to said at least one noble gas, less than 50% by volume of oxygen, carbon dioxide or a mixture thereof.

9. The method according to claim 8, wherein said admixture contains, in addition to said at least one noble gas, less than 30% by volume of oxygen, carbon dioxide or a mixture thereof.

10. The method according to claim 9, wherein said admixture contains, in addition to said at least one noble gas, less than 20% by volume of oxygen, carbon dioxide or a mixture thereof.

11. The method according to claim 10, wherein said admixture contains, in addition to said at least one noble gas, less than 10% by volume of oxygen, carbon dioxide or a mixture thereof.

12. The method according to claim 1, wherein said at least one noble gas is about 90% to 99% by volume of argon and 1% to 10% by volume of Xe or Kr or both.

13. The method according to claim 2, wherein said admixture contains about 50% by volume of Ne and 50% by volume of He.

14. The method according to claim 2, wherein said at least one noble gas comprises about 5% to 10% by volume of Xe and 90% to 95% by volume of Kr.

15. The method according to claim 2, wherein said admixture contains less than 2% by volume of argon, oxygen, nitrogen or a mixture thereof.

16. The method according to claim 1, wherein the temperature at which said at least one enzyme in said sensor is contacted with said at least one noble gas is between 0°

C. and 40°

C.

17. The method according to claim 1, wherein the temperature at which said at least one enzyme in said sensor is contacted with said at least one noble gas is between 10°

C. and 30°

C.

18. The method according to claim 1, wherein the pressure of the at least one noble gas is less than 10 atmospheres.

19. The method according to claim 18, wherein the pressure of the at least one noble gas is less than 3 atmospheres.

20. The method according to claim 19, wherein the pressure of the at least one noble gas is between 1 and 2 atmospheres.

21. The method according to claim 20, wherein the pressure of said at least one noble gas is about 1 atmosphere.

22. The method of claim 1, which comprises passing said at least one noble gas into a reaction space or medium from through said semi-permeable membrane, said reaction space or medium containing said at least one enzyme;

allowing said at least one noble gas effect substantial saturation of the reaction space or medium; and

maintaining said substantial saturation for a time sufficient for said at least one enzyme to detect said at least one noble gas.