Anti-focosylceramide monoclonal antibody

• US 5,331,093 A
• Filed: 05/27/1992
• Issued: 07/19/1994
• Est. Priority Date: 11/09/1988
• Status: Expired due to Term

• Current Assignee: Hoechst Japan Limited
• Original Assignee: Hoechst Japan Limited
• Inventors: Ishihara, Hideki, Hattori, Hiroshi, Ono, Kenichi
• Primary Examiner(s): Hutzell, Paula
• Assistant Examiner(s): LORING, SUSAN
• Application Number:
  US07/888,306
• Time in Patent Office: 783 Days
• Field of Search: 530/388.1, 530/388.8, 530/387.5, 435/240.27, 435/172.2, 435/70.21, 435/7.23
• US Class Current: 530/387.5
• CPC Class Codes: C07K 16/30 from tumour cells

Abstract

The invention relates to a monoclonal antibody PC47H specifically recognizing fucosylceramide derived from a ceramide-mono-glycoside fraction of neutral glycolipid extracted and purified from human cancer tissues and having properties such as belonging to IgM and showing reactivity to neither of normal peripheral blood lymphocyte, normal erythrocyte, normal fibroblast nor cell lines derived from leukemia, hepatoma, breast cancer and neuroblastoma but to cell lines derived from lung cancer, stomach cancer, colon cancer and pancreas cancer; a hybridoma having ability to produce the aforementioned monoclonal antibody; a method for manufacturing the aforementioned monoclonal antibody which comprises immunizing an animal with a neutral glycolipid fraction extracted from human pancreas cancer, fusing animal cells with myeloma cells to generate hybridomas, cloning the hybridomas, selecting clones which produce monoclonal antibodies which specifically recognize fucosylceramide and then using the clones to manufacture the monoclonal antibody; and a diagnostic aid of cancers such as lung cancer, stomach cancer, colon cancer and pancreas cancer containing the aforementioned monoclonal antibody as an active component.

Claims

1. A monoclonal antibody which is PC47H as produced by the hybridoma deposited under international deposit number FERM BP-2557.

2. A diagnostic acid for lung, colon or stomach cancer, said diagnostic aid comprising a monoclonal antibody as claimed in claim 1 and a carrier.