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Method, system and reagents for DNA sequencing

  • US 5,332,666 A
  • Filed: 10/22/1991
  • Issued: 07/26/1994
  • Est. Priority Date: 07/02/1986
  • Status: Expired due to Term
First Claim
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1. A process of DNA sequence analysis by chain elongation comprising reacting a template of DNA contained in an appropriate sequencing vector, a primer, a polymerase, a first mixture of DNA nucleotides or their analogs, and a second mixture of radiation omitting reporter-labeled chain terminators corresponding to the DNA nucleotides or their analogs to produce fragments of DNA having reporters covalently attached to their 3'"'"' terminal residues introduced by chain termination events, and analyzing the fragments so produced for the presence of reporters, thereby identifying the DNA sequence.

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