Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
First Claim
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1. A method of electrochemical determination of an analyte comprising:
- obtaining a bioelectrode prepared from a colloidal gold adsorbed peroxidase and an oxidase in communication with a conducting surface; and
detecting current generated in the presence of the analyte when a sample containing the analyte is contacted with the bioelectrode.
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Abstract
Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytyes depending on the oxidase employed.
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20 Claims
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1. A method of electrochemical determination of an analyte comprising:
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obtaining a bioelectrode prepared from a colloidal gold adsorbed peroxidase and an oxidase in communication with a conducting surface; and detecting current generated in the presence of the analyte when a sample containing the analyte is contacted with the bioelectrode. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for mediatorless electrochemical determination of glucose, comprising the steps:
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obtaining a bioelectrode prepared from colloidal gold adsorbed horseradish peroxidase and glucose oxidase in communication with a conducting surface; and detecting current produced from reduction of hydrogen peroxide generated from glucose oxidase catalyzed glucose conversion. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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- 15. A method of determining glucose concentration wherein the glucose is electrochemically determined from a current generated from reduction of hydrogen peroxide produced by glucose oxidase catalyzed glucose conversion, the glucose oxidase being immobilized on a conducting surface with colloidal gold adsorbed horseradish peroxidase.
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