Process and agent for the colorimetric determination of an analyte by means of enzymatic oxidation
First Claim
1. A composition for use in the colorimetric determination of an analyte comprisinga) an oxidoreductase enzyme dependent from the cofactors flavine or PQQ which is effective to oxidize the analyte;
- b) an aromatic nitroso compound or a tautomerically equivalent oxime compound which is effective to accept electrons from the enzyme/cofactor to cause a color change in the reaction mixture; and
c) a coupling reagent for an oxidative coupling reaction.
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Abstract
The present invention provides a process for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination of the reduced electron acceptor by color formation as measure for the amount of the analyte, wherein the analyte is oxidized with an appropriate oxidoreductase in the presence of a substance selected from the group of compounds with nitrogen in an oxidation stage between +1 and -1 as direct electron acceptor.
The present invention also provides an agent for the colorimetric determination of an analyte by enzymatic oxidation of the analyte, containing an oxidoreductase and a color-forming electron acceptor, wherein the color-forming electron acceptor is a substance reacting directly with the analyte/enzyme system selected from the group of compounds with nitrogen in an oxidation stage between +1 and -1.
57 Citations
20 Claims
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1. A composition for use in the colorimetric determination of an analyte comprising
a) an oxidoreductase enzyme dependent from the cofactors flavine or PQQ which is effective to oxidize the analyte; -
b) an aromatic nitroso compound or a tautomerically equivalent oxime compound which is effective to accept electrons from the enzyme/cofactor to cause a color change in the reaction mixture; and c) a coupling reagent for an oxidative coupling reaction. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A composition for use in the colorimetric determination of an analyte comprising
a) an oxidoreductase enzyme dependent from the cofactors flavine or PQQ which will oxidize the analyte; -
b) an aromatic nitroso compound or a tautomerically equivalent oxime compound which will accept electrons from the enzyme/cofactor to cause a color change in the reaction mixture; and c) a substance which forms a color by transition from the oxidized form into the reduced form. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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15. A composition for use in the colorimetric determination of an analyte comprising
a) an oxidoreductase enzyme dependent from the cofactor PQQ which will oxidize the analyte; b) an aromatic nitroso compound or a tautomerically equivalent oxime compound which will accept electrons from the enzyme/cofactor to cause a color change in the reaction mixture. - View Dependent Claims (16, 17, 18, 19, 20)
Specification