Detection of biological macromolecules by NMR-sensitive labels
First Claim
1. An apparatus for detecting electrophoretically separated biological molecules having at least one nuclear magnetic resonance-sensitive isotope comprising:
- a) a magnet for providing a magnetic field of at least 1000 gauss at a predetermined sample area;
b) excitation means for exciting at least three nuclear magnetic resonance-sensitive isotopes of said separated biological molecules at said predetermined sample area;
c) detection means for detecting and identifying which of said at least three nuclear magnetic resonance-sensitive isotopes are present in the separated biological molecules at said predetermined sample area; and
d) an electrophoresis column positioned to deliver the separated biological molecules to the sample area where at least one nuclear magnetic-sensitive isotope is subjected to the magnetic field of the magnet, excited by the excitation means, and detected and identified by the detection means.
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Abstract
An apparatus and method for identifying the presence of biological molecules labeled with isotopes that are identifiable and distinguishable by their responses to nuclear magnetic resonance. The method features the use of labels that are:
1) not radioactive or only weakly radioactive, therefore not hazardous to handle, and have a long storage life.
2) small in molecular weight, thus not significantly changing the electrophoresis migration rate of the labeled material.
3) available as several distinguishable labels, thereby enabling simultaneous detection of several classes of molecules.
57 Citations
10 Claims
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1. An apparatus for detecting electrophoretically separated biological molecules having at least one nuclear magnetic resonance-sensitive isotope comprising:
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a) a magnet for providing a magnetic field of at least 1000 gauss at a predetermined sample area; b) excitation means for exciting at least three nuclear magnetic resonance-sensitive isotopes of said separated biological molecules at said predetermined sample area; c) detection means for detecting and identifying which of said at least three nuclear magnetic resonance-sensitive isotopes are present in the separated biological molecules at said predetermined sample area; and d) an electrophoresis column positioned to deliver the separated biological molecules to the sample area where at least one nuclear magnetic-sensitive isotope is subjected to the magnetic field of the magnet, excited by the excitation means, and detected and identified by the detection means. - View Dependent Claims (2, 3, 4, 5)
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6. A method for use with electrophoresis to analyze a mixture of biological molecules comprising the steps of:
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a) labeling said mixture of biological molecules with nuclear magnetic resonance-sensitive isotopes such that the biological molecules have at least one of at least three nuclear magnetic resonance-sensitive isotopes; b) separating said mixture of biological molecules on an electrophoresis column to form bands of separated biological molecules; c) exposing said separated biological molecules to a magnetic field of at least 1000 gauss; d) providing means for exciting each of said at least three nuclear magnetic resonance-sensitive isotopes while said separated biological molecules are in said magnetic field; and e) detecting and identifying which of said at least three nuclear magnetic resonance-sensitive isotopes are present in the separated biological molecules in said magnetic field. - View Dependent Claims (7, 8, 9, 10)
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Specification