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Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method

  • US 5,340,722 A
  • Filed: 03/29/1993
  • Issued: 08/23/1994
  • Est. Priority Date: 08/24/1988
  • Status: Expired due to Fees
First Claim
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1. A method for the continuous and reversible determination of the concentration of an enzyme substrate in a specimen, comprising the steps of:

  • (a) contacting said specimen containing said enzyme substrate with an enzyme specific for said substrate, selected from the group consisting of oxidases and oxygenases, to which a flavin coenzyme selected from the group consisting of flavin mononucleotide and flavin adenine dinucleotide is bonded, said flavin coenzyme converting to a reduced form by said enzyme substrate and to an oxidized form by molecular oxygen dissolved in said specimen,(b) measuring a change in intrinsic fluorescence of said flavin coenzyme under fluorescent excitation resulting from reduction of said flavin coenzyme occurring in step (a), and(c) determining the concentration of enzyme substrate in said specimen from the change in intrinsic fluorescence measured in step (b).

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