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Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process

  • US 5,340,740 A
  • Filed: 05/15/1992
  • Issued: 08/23/1994
  • Est. Priority Date: 05/15/1992
  • Status: Expired due to Term
First Claim
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1. A method of producing a sustained culture of undifferentiated avian cells expressing an embryonic stem cell phenotype, comprising:

  • collecting avian cells from an avian blastoderm prior to formation of the primitive streak;

    depositing said avian cells in contact with a mouse fibroblast feeder cell layer;

    growing said avian cells on said mouse fibroblast feeder cell layer in the presence of a media containing leukemia inhibitory factor in a differentiation-inhibiting amount for a time sufficient to produce a sustained avian cell culture;

    said sustained avian cell culture consisting essentially of undifferentiated avian cells expressing an embryonic stem cell phenotype.

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