Oncofetal gene, gene product and uses therefor

• US 5,342,761 A
• Filed: 10/01/1990
• Issued: 08/30/1994
• Est. Priority Date: 10/01/1990
• Status: Expired due to Fees

• Current Assignee: Research & Development Foundation
• Original Assignee: Research & Development Foundation
• Inventors: MacLeod, Carol L.
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): NISBET, THOMAS
• Application Number:
  US07/590,894
• Time in Patent Office: 1,429 Days
• Field of Search: 435/69.1, 435/69.3, 435/320.1, 435/172.3, 530/350, 536/23.1, 424/88
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/82 Translation products from oncogenes
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The present invention provides a novel cDNA sequence (Pem), a gene product protein (Seq. id No. 1), and uses for this novel cDNA Sequence and the Pem gene product. The DNA sequence and recombinant DNA molecules of this invention are characterized in that each codes for a novel protein having the following characteristics: (1) is expressed by T lymphoma cells, (2) is not expressed in normal thymus, activated spleen cells, gut associated lymphoid tissue, or bone marrow, and is not detectable in adult brain, liver, large intestine or ovary, (3) is expressed in immortalized or cancerous cell lines, and (4) is expressed in embryonic development.

Claims

1. A cDNA probe comprising the nucleotide sequence shown in Seq. id No. 1.

2. An expression vehicle which comprises a DNA sequence coding for the Pem (Seq. id. No. 1) protein, wherein said expression vehicle is capable of replication in a host which comprises, in operable linkage;

a) an origin of replication;

b) a promoter; and

c) a DNA sequence coding for Pem (Seq. id. No.

     1) protein.

3. The expression vehicle of claim 2, wherein said expression vehicle is a plasmid capable of replication in a host which comprises, in operable linkage:

a) an origin of replication;

b) a promoter; and

c) a DNA sequence coding for Pem (Seq. id. No.

     1) protein.

4. The expression vehicle of claim 3, wherein said expression vehicle is a plasmid capable of replication in a prokaryotic host which comprises, in operable linkage:

a) a prokaryotic origin of replication;

b) a prokaryotic promoter; and

c) a DNA sequence coding for Pem (Seq. id. No.

     1) protein.

5. A vector comprising a DNA sequence coding for Pem (Seq. id. No. 1) protein, wherein said vector is capable of replication in a host which comprises, in operable linkage;

a) an origin of replication;

b) a promoter; and

c) a DNA sequence coding for Pem (Seq. id. No.

     1) protein.

6. The vector of claim 5 wherein said vector is isolated from the group consisting of a plasmid, a phage and a cosmid.

7. The vector of claim 6, wherein said vector is pT7T3-20.2 having ATCC Accession number 68304.

8. A host transformed with a recombinant DNA molecule wherein said recombinant DNA molecule comprises a DNA sequence coding for the Pem (Seq. id. No. 1) protein.

9. The host of claim 8 which is E. coli.