Method for determining pyrogen content
First Claim
1. A method for the detection of the presence of a pyrogen in a specimen, which comprises bringing the specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound said compound being histidine, or adenine, bonded to agarose through hexamethylenediamine, and, without eluting the pyrogen adsorbed ont he adsorbent, detecting whetehr there is any adsorbed pyrogen on the adsorbent by directly subjecting the adsorbent to a Limulus test.
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Abstract
A method for determination of a pyrogen content which comprises bringing a specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound bonded to a water-insoluble carrier directly or through a spacer, and after or without eluting the pyrogen adsorbed on the adsorbent, determining the content of the adsorbed pyrogen by the Limulus method.
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Citations
5 Claims
- 1. A method for the detection of the presence of a pyrogen in a specimen, which comprises bringing the specimen into contact with an adsorbent composed of a nitrogen-containing heterocyclic compound said compound being histidine, or adenine, bonded to agarose through hexamethylenediamine, and, without eluting the pyrogen adsorbed ont he adsorbent, detecting whetehr there is any adsorbed pyrogen on the adsorbent by directly subjecting the adsorbent to a Limulus test.
Specification
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- Resources
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Current AssigneeTanabe Seiyaku Co Ltd (Mitsubishi Chemical Group Corp.)
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Original AssigneeTanabe Seiyaku Co Ltd (Mitsubishi Chemical Group Corp.)
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InventorsSato, Tadashi, Minobe, Satoshi, Tosa, Tetsuya, Watanabe, Taizo
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Primary Examiner(s)ALEXANDER, LYLE
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Application NumberUS08/141,689Time in Patent Office308 DaysField of Search436/86, 436/501-502, 436/178, 435/34, 210/651, 210/692US Class Current436/178CPC Class CodesG01N 33/579 involving limulus lysateY10T 436/255 including use of a solid so...
