Dielectrophoretic characterization of micro-organisms and other particles

• US 5,344,535 A
• Filed: 04/27/1993
• Issued: 09/06/1994
• Est. Priority Date: 11/27/1989
• Status: Expired due to Term

• Current Assignee: British Technology Group Ltd.
• Original Assignee: British Technology Group Ltd.
• Inventors: Betts, Walter B., Hawkes, Jeremy J.
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US08/053,326
• Time in Patent Office: 497 Days
• Field of Search: 204/299 R, 204/180.1, 204/183.1, 204/183.3
• US Class Current: 204/547
• CPC Class Codes: B03C 5/026 using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
• CPC Class Codes: C12M 41/30 of concentration
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence

Abstract

Dielectrophoretic collection rates of micro-organisms or other animate or inanimate dielectrically polarizable particles in suspension in a fluid are established by flowing the suspension past electrodes energized to produce a non-uniform alternating electric field in the suspension, terminating the energization of the electrodes after a predetermined time to release particles collected from the suspension, and, downstream of the electrodes, measuring the pulse of released particles as a measure of the rate of particle collection during energization of the electrodes. Repeated measurements at different field frequencies enable a collection-rate spectrum to be established and the particles under examination to be characterized or identified by reference to known spectra of known particles.

Claims

1. Apparatus for establishing dielectrophoretic collection rates for particles in a fluid suspension, said apparatus comprising:

a chamber for said fluid suspension, said chamber having an inlet and an outlet;

an electrode system comprising closely spaced interdigitated electrodes for influencing said fluid suspension within said chamber;

means for applying an alternating voltage between said interdigitated electrodes of said electrode system and thereby establishing in said fluid suspension in the immediate vicinity of said electrodes a spatially non-uniform alternating electric field thereby to induce dielectrophoretic collection adjacent said electrodes of electrically polarizable particles suspended in said fluid suspension;

means for producing fluid flow of said fluid suspension through said chamber; and

means for measuring particle concentration at a measuring region within said chamber, said inlet and outlet being so disposed that fluid suspension flowing through said chamber from said inlet to said outlet flows past said electrode system and immediately thereafter past said measuring region within said chamber.

2. Apparatus as claimed in claim 1, wherein said means for producing fluid flow in fluid circulating means for re-circulating fluid from said outlet to said inlet of said chamber.

3. Apparatus as claimed in claim 1, wherein said means for measuring particle concentration comprises a light source arranged to project a beam of light through said chamber at said measuring region, immediately downstream of said electrode system, and light detector means sensitive to the intensity of said light beam after transmission through said chamber and thus to increased or decreased absorption or scattering of said light beam which indicates an increase or decrease in concentration of particles suspended by said fluid suspension traversed by said light beam at said measuring region.

4. A method of establishing dielectrophoretic collection rates for dielectrically polarizable particles in a fluid suspension, said method comprising the steps of:

causing a fluid suspension to flow in a chamber past an electrode structure;

energizing said electrode structure for a predetermined time interval with an alternating voltage at a preselected frequency and thereby establishing in said flowing fluid suspension a spatially non-uniform alternating electrical field and inducing dielectrophoretic collection, adjacent said electrode structure, of dielectrically polarizable particles suspended in said fluid suspension;

thereafter terminating the energizing of said electrode structure and thereby releasing particles collected adjacent thereto as a localized pulse of increased particle concentration in the flowing fluid suspension; and

measuring said pulse of increased particle concentration at a measuring region located within said chamber and immediately downstream of said electrode structure.

5. A method of establishing a dielectrophoretic collection-rate spectrum for particles in a fluid suspension, said method comprising carrying out the method of claim 4 repeatedly, using different frequencies for the applied alternating voltage during successive electrode structure energization intervals to produce data required for establishing either entire or critical ranges of the collection-rate spectrum of the fluid suspension.

6. A method of identifying dielectrically polarizable particles contained in a fluid suspension, said method comprising establishing a dielectrophoretic collection-rate spectrum data therefor according to the method as claimed in claim 5 and correlating said data thus acquired with corresponding known data relating to the collection-rate spectra of particular identified particles, to establish relative or absolute concentrations of such identified particles required to be present in the suspension under examination in order that it should yield the collection-rate data which have been acquired.