Process for producing filtration membranes having amino acids that are capable of adsorbing proteins

• US 5,344,560 A
• Filed: 10/12/1993
• Issued: 09/06/1994
• Est. Priority Date: 05/10/1990
• Status: Expired due to Fees

• Current Assignee: Japan Atomic Energy Research Institute
• Original Assignee: Japan Atomic Energy Research Institute
• Inventors: Saito, Kyoichi, Sugo, Takanobu
• Primary Examiner(s): SPEAR JR, FRANK
• Application Number:
  US08/135,423
• Time in Patent Office: 329 Days
• Field of Search: 427/245, 210/500.23, 210/500.35, 210/632, 210/638, 521/27, 521/32, 502/402
• US Class Current: 210/500.23
• CPC Class Codes: B01D 15/00 Separating processes involving the treatment of liquids with solid sorbents using liquid sorbents B01D11/00; ion exchange processes or materials, sorbent materials in general B01J, e.g. sorbents for chromatography B01J20/281; for investigating or analysing materials G01N30/00; Apparatus therefor
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: B01D 2323/385 involving radiation
• CPC Class Codes: B01D 67/0093 Chemical modification
• CPC Class Codes: B01D 67/00931 by introduction of specific groups after membrane formation, e.g. by grafting
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/264 derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
• CPC Class Codes: B01J 20/265 modified or post-treated polymers polymer carriers or substrates subjected to further impregnating or coating B01J20/3208
• CPC Class Codes: B01J 20/28038 Membranes or mats made from fibers or filaments
• CPC Class Codes: B01J 20/28078 Pore diameter
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3278 Polymers being grafted on the carrier
• CPC Class Codes: B01J 20/328 Polymers on the carrier being further modified

Abstract

There is described a process for producing a filtration membrane that is also capable of adsorbing proteins. The process comprises the steps of subjecting a porous polymeric substrate to a graft polymerization reaction with a polymerizable monomer under exposure to an ultraviolet radiation or an ionizing radiation and thereafter bringing an amino acid into contact with the resulting graft membrane for immobilization. The amino acid is contained in an amount of 0.01-1 millimole per gram of the porous polymeric substrate. The polymerizable monomer to be grafted onto the porous polymeric substrate contains an epoxy group.

Claims

1. A process for producing a filtration membrane that is also capable of adsorbing proteins, comprising:

subjecting a porous polymeric substrate made of a homo- or copolymer of an olefin or a halogenated olefin and a polymerizable epoxy group containing monomer to a graft polymerization reaction to form graft chains on the substrate, said graft polymerization reaction occurring upon exposure to ultraviolet or ionizing radiation, and

immobilizing an amino acid to the graft chains by forming a covalent bond between the epoxy group of the graft chains and the amino group of the amino acid.

2. A process according to claim 1 wherein said porous polymeric substrate is in the form of either a flat membrane or an assembly of hollow fibers and has an average pore size of 0.01-10 μ

m.

3. A process according to claim 1 wherein the amino acid is contained in an amount of 0.01-1 millimole per gram of the porous polymeric substrate.

4. A process according to claim 1 wherein the polymerizable epoxy group-containing monomer is selected from the group consisting of glycidyl methacrylate, glycidyl acrylate, glycidyl metaitaconate, ethylglydicyl maleate and glycidyl vinyl sulfonate.

5. A process according to claim 1 wherein the amino acid is selected from the group consisting of phenylalanine, tryptophan and histidine.

6. A process according to claim 1 wherein the homopolymer or halogenated homopolymer is selected from the group consisting of polyethylene, polypropylene and polytetrafluoroethylene, and wherein the copolymer or halogenated copolymer is a copolymer of monomers selected from the group consisting of ethylene, propylene, tetrafluoroethylene and chlorotrifluoroethylene.

7. The process of claim 1 wherein the amino acid is immobilized on the graft membrane by contacting the graft membrane with an aqueous solution of amino acids.

8. A filtration membrane prepared by a process comprising:

subjecting a porous polymeric substrate made of a homo- or copolymer of an olefin or a halogenated olefin and a polymerizable epoxy group-containing monomer to a graft polymerization reaction to form graft chains on the substrate, said graft polymerization reaction occurring upon exposure to ultraviolet or ionizing radiation, and

immobilizing an amino acid to the graft chains by forming a covalent bond between the epoxy group of the graft chains and the amino group of the amino acid.

9. A filtration membrane according to claim 8 wherein said porous polymeric substrate is in the form of a flat membrane or an assembly of hollow fibers and has an average pore size of 0.01-10 μ

m.

10. A filtration membrane according to claim 8 wherein the amino acid is contained in an amount of 0.01-1 millimole per gram of the porous polymeric substrate.

11. A filtration membrane according to claim 8 wherein the polymerizable epoxy group-containing monomer is selected from the group consisting of glycidyl methacrylate, glycidyl acrylate, glycidyl metaitaconate, ethylglydicyl maleate and glycidyl vinyl sulfonate.

12. A filtration membrane according to claim 8 wherein the amino acid is selected from the group consisting of phenylalanine, tryptophan and histidine.

13. A filtration membrane according to claim 8 wherein the homopolymer or halogenated homopolymer is selected from the group consisting of polyethylene, polypropylene and polytetrafluoroethylene and wherein the copolymer or halogenated copolymer is a copolymer of monomers selected from the group consisting of ethylene, propylene, tetrafluoroethylene and chlorotrifluoroethylene.