Assay timed by electrical resistance change and test strip

• US 5,344,754 A
• Filed: 01/13/1993
• Issued: 09/06/1994
• Est. Priority Date: 01/13/1993
• Status: Expired due to Term

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Avocet Medical, Inc. (Danaher Corporation)
• Inventors: Zweig, Stephen E.
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): GITOMER, RALPH J
• Application Number:
  US08/003,771
• Time in Patent Office: 601 Days
• Field of Search: 435/4, 435/817, 436/46, 436/805, 436/806, 436/808, 422/56, 204/153.12
• US Class Current: 435/4
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/56 involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
• CPC Class Codes: G01N 27/041 of a solid body
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10T 436/112499 with sample on test slide

Abstract

Assays for liquid analytes are performed on a bibulous matrix containing dried reagents which produce a visibly detectable reaction product. Application of liquid sample to the bibulous matrix is detected by measuring a drop in resistance across the matrix. A preferred test article for performing the method includes the matrix and a pair of spaced-apart electrodes in electrical contact with a reaction zone on the matrix. The test article is used in combination with a detection unit having means for probing the electrodes to determine when electrical resistance in the matrix has decreased. The assay methods and apparatus are particularly useful for performing enzyme assays where signal developed as a function of time is critical.

Claims

1. An assay for determining an analyte in a liquid sample, said assay comprising:

applying a volume of the sample to a target location on a bibulous matrix, wherein the matrix comprises one or more dried reagents which in the presence of the analyte initiate time-dependent production of an optically detectable signal within the matrix;

measuring a change in electrical resistance across the target location which results from the application of liquid sample;

initiating a timing cycle when a lowering of electrical resistance is first measured; and

measuring the production of said optically detectable signal within the matrix at one or more times during the timing cycle.

2. An assay as in claim 1 wherein the sample volume is from 3 μ

l to 50 μ

l.

3. An assay as in claim 1, wherein the sample is a patient sample.

4. An assay as in claim 3, wherein the patient sample is blood.

5. An assay as in claim 1, wherein the analyte is an enzyme and the reagents include an enzyme substrate which produces an optically detectable change after exposure to the enzyme.

6. An assay as in claim 5 wherein the optically detectable change is fluorescence, luminescence, or color change.

7. An assay as in claim 5, wherein the analyte is a component of a coagulation pathway and the enzyme substrate produces the detectable signal upon activation by said component.

8. An assay as in claim 1, further comprising controlling the temperature of the bibulous matrix to 37°

C.

9. An assay as in claim 1, further comprising continuously monitoring the electrical resistance across the target location, whereby loss of liquid sample can be detected.

10. An assay as in claim 1, wherein the production of signal is detected at a plurality of time points over a preselected time duration.

11. An improved enzyme assay of the type wherein a liquid sample suspected of containing the enzyme is applied to a bibulous matrix-containing reagents which result in production of an optically detectable signal over time, wherein the improvement comprises measuring a change in electrical resistance within the matrix resulting from application of the liquid sample and optically detecting the production of signal at one or more times after the electrical resistance change is first measured.

12. An improved assay as in claim 11, wherein electrical resistance is measured by contacting a pair of electrodes which are secured to the bibulous matrix on either side of a location where liquid sample is applied.

13. An improved assay as in claim 11, wherein the electrical resistance is continuously monitored to assure that the liquid sample has not been lost during the assay procedure.

14. An improved assay as in claim 11, wherein the production of signal is detected at a plurality of time points over a preselected time duration which commences when the change in electrical resistance is first measured.

15. A test article comprising:

a bibulous matrix;

one or more dried reagents which are present within the matrix and which, when wetted by a liquid sample, initiate an optically detectable chemical reaction with an analyte present in the sample; and

a pair of spaced-apart electrodes proximate a sample target location of the matrix, wherein the electrodes are disposed so that application of the liquid sample to the target location will lower electrical resistance between the electrodes.

16. A test article as in claim 15, wherein the spaced-apart electrodes are secured to the bibulous matrix and in electrical contact with at least a portion of a surface of the matrix on opposite sides of the target location.

17. A test article as in claim 16, wherein the electrodes are metal foil strips which are attached to the matrix surface by an adhesive.

18. A test article as in claim 15, wherein the gap between the spaced-apart electrodes has a width in the range from 0.1 mm to 2 mm, whereby the electrode inhibits evaporation of sample from the matrix.

19. A test article as in claim 18, further comprising a transparent support element attached to a surface of the matrix opposite to the electrodes.

20. A test article as in claim 15, wherein the bibulous matrix is composed of a hydrophilic, non-swellable material which is free from interference with a coagulation pathway.

21. A test article as in claim 20, wherein the reagents comprise a coagulation initiator and a substrate which produces a detectable signal upon activation by a component of the coagulation pathway.

22. A test article as in claim 21, wherein the coagulation pathway component is thrombin and the substrate is a peptide cleavably bound to a reporter molecule, wherein thrombin binds the peptide and cleaves the reporter molecule to produce the detectable signal.

23. A test article as in claim 22, wherein the detectable signal is fluorescence.

24. A test article comprising:

a bibulous matrix;

one or more dried reagents which are dispersed within the matrix and which, when wetted by a liquid sample, initiate an optically detectable enzymatic reaction with an enzyme analyte present in the sample; and

a pair of spaced-apart metal strips attached to a surface of the matrix to define a sample target location therebetween, whereby application of the liquid sample to the target location will lower electrical resistance between the metal strips.

25. A test article as in claim 24, where the metal strips are attached to the matrix surface by an adhesive.

26. A test article as in claim 24, wherein the gap between the spaced-apart metal strips has a width in the range from 0.1 mm to 2 mm, whereby the strips inhibit evaporation of sample from the matrix.

27. A test article as in claim 26, further comprising a transparent support element attached to a surface of the membrane opposite to the metal strips.

28. A test article as in claim 24, wherein the bibulous matrix is composed of a hydrophilic, nonswellable material which is free from interference with a coagulation pathway.

29. A test article as in claim 28, wherein the reagents comprise a coagulation initiator and a substrate which produces a detectable signal upon activation by a component of the coagulation pathway.

30. A test article as in claim 29, wherein the coagulation initiation is thrombin and the substrate is a peptide cleavably bound to a reporter molecule, wherein thrombin binds the peptide and cleaves the reporter molecule to produce the detectable signal.

31. A test article as in claim 30, wherein the detectable signal is fluorescence.