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Devices for containing biological specimens for thermal processing

  • US 5,346,672 A
  • Filed: 03/02/1993
  • Issued: 09/13/1994
  • Est. Priority Date: 11/17/1989
  • Status: Expired due to Fees
First Claim
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1. A chamber device for detecting molecular targets in a biological specimen at temperatures elevated above ambient temperature, comprising:

  • (a) a microscope slide having a flat specimen area containing a biological specimen;

    (b) a cover covering said flat specimen area;

    said cover comprising a flange which sealingly contacts the microscope slide about the flat specimen area, a ceiling connected to the flange and a neck which protrudes upwardly from the ceiling;

    (c) said cover and said flat specimen area forming an enclosure having an interior space comprising a chamber device that prevents evaporative loss of reaction fluids;

    (d) said neck in said cover having a channel for adding reaction fluids to said chamber device and removing reaction fluids from said chamber device after said enclosure is formed; and

    (e) means for closing said channel to provide a water vapor barrier in said chamber device.

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