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Homogeneous amperometric immunoassay

  • US 5,346,832 A
  • Filed: 12/18/1992
  • Issued: 09/13/1994
  • Est. Priority Date: 06/09/1989
  • Status: Expired due to Fees
First Claim
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1. A method for determining the concentration of an analyte present in a sample of biological fluid contained in an electrochemical cell equipped with a set of electrodes, said method comprising the steps of:

  • a) forming an electroactive complex containing an antigenic analyte chemically bonded to a carrier molecule, said carrier molecule also being chemically bonded to an electroactive molecule capable of transferring a charge to an electrode;

    b) adding the biological fluid, predetermined amounts of the electroactive complex, and an antibody to the analyte to the cell;

    c) applying a voltage across the electrodes and measuring the current in the fluid at different voltage levels to form a voltage/current relationship for the sample fluid; and

    d) comparing the voltage/current relationship for the sample fluid with standard voltage/current relationships generated using known amounts of analytes to determine the concentration of analyte present in the sample.

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