Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex

• US 5,346,990 A
• Filed: 03/12/1991
• Issued: 09/13/1994
• Est. Priority Date: 04/08/1987
• Status: Expired due to Term

• Current Assignee: Cytogam Incorporated
• Original Assignee: Cytogam Incorporated
• Inventors: Spaulding, Glenn F.
• Primary Examiner(s): Schain, Howard E.
• Assistant Examiner(s): TOUZEAU, LYNN
• Application Number:
  US07/667,974
• Time in Patent Office: 1,281 Days
• Field of Search: 435/2, 530/350, 530/387, 530/852, 530/853, 530/388.24, 530/388.2, 530/389.1, 530/387.1, 424/105, 424/537, 424/85.9
• US Class Current: 530/350
• CPC Class Codes: A61K 35/52 Sperm; Prostate; Seminal fluid; Leydig cells of testes
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: G01N 2800/367 Infertility, e.g. sperm disorder, ovulatory dysfunction
• CPC Class Codes: G01N 33/689 related to pregnancy or the gonads
• CPC Class Codes: Y10S 424/811 involving sex selection or contraception
• CPC Class Codes: Y10S 435/806 Fertility tests
• CPC Class Codes: Y10S 436/906 Fertility tests
• CPC Class Codes: Y10S 530/852 Sperm

Abstract

A method of sorting living cells based on DNA content. Mammalian sperm subpopulations enriched in X- or Y-sperm. X- and Y-enriched sperm-plasma-membrane vesicles. Substantially pure sex-associated membrane (SAM) proteins. Antibodies binding to X-or Y-SAM proteins, essentially free of antibodies binding to Y- or X-SAM proteins respectively, or to the H-Y antigen. Semen samples enriched for X- or Y-sperm. Methods for increasing the probability that offspring will be male or female comprising the step of allowing as penn from an enriched semen sample to fertilize an ovum. Methods for increasing the probability that offspring will be male or female comprising the step of immunizing a female with X- or Y-SAM proteins. Methods of decreasing fertility comprising the step of immunizing a female with both X- and Y-sperm. Methods of increasing the probability that mammalian offspring will carry a gene for a particular sex-chromosome linked trait.

Claims

1. A refined X sex-associated membrane protein characterized by a higher band density on an SDS/PAGE of plasma membrane proteins prepared from X-enriched sperm subpopulations as compared to the corresponding band density on an SDS/PAGE of plasma membrane proteins prepared from whole sperm or prepared from Y-enriched sperm subpopulations;

or a refined Y sex-associated membrane protein characterized by a higher band density on an SDS/PAGE of plasma membrane proteins prepared from Y-enriched sperm subpopulations as compared to the corresponding band density on an SDS/PAGE of plasma membrane proteins prepared from whole sperm or prepared from X-enriched sperm subpopulations.

5. The refined X sex-associated membrane protein according to claim 1.

6. The refined Y sex-associated membrane protein according to claim 1.

2. A substantially pure X sex-associated membrane protein characterized by a higher band density on an SDS/PAGE an a higher spot density on an IPG-SDS/PAGE of plasma membrane proteins prepared from X-enriched sperm subpopulations as compared to the corresponding band density on an SDS/PAGE and the corresponding spot density on an IPG-SDS/PAGE of plasma membrane proteins prepared from whole sperm or prepared from Y-enriched sperm subpopulations;

or a substantially pure Y sex-associated membrane protein characterized by a higher band density on SDS/PAGE and a higher spot density on IPG-SDS/PAGE of plasma membrane proteins prepared from Y-enriched sperm subpopulations as compared to the corresponding band density on an SDS/PAGE and the corresponding spot density on an IPS-SDS/PAGE of plasma membrane proteins prepared from whole sperm or prepared from X-enriched sperm subpopulations.

3. The X sex-associated membrane protein according to claim 2 selected from the group consisting of proteins having a molecular weight (KD) and pI as follows:

(1) 20.9, 5.74;

(2) 26.3, 7.58;

(3) 27.8, 6.08;

(4) 44.1, 6.90;

(5) 52.5, 5.33;

(6) 58.0, 5.99;

(7) 59.4, 6.59;

(8) 59.5, 6.81;

(9) 62.1, 7.23;

(10) 62.5, 5.54;

(11) 62.7, 6.85;

(12) 62.8, 6.64;

(13) 63.9, 5.83;

(14) 68.2, 5.95; and

(15) 78.6, 7.14.

4. The Y sex-associated membrane protein according to claim 2 selected from the group consisting of proteins having a molecular weight (KD) and pI as follows:

(1) 9.6, 6.58;

(2) 19.9, 5.67;

(3) 29.0, 6.67;

(4) 36.5, 7.16;

(5) 41.1, 6.21;

(6) 55.5, 6.82;

(7) 55.9, 5.25;

(8) 58.0, 8.67;

(9) 62.9, 6.34; and

(10) 70.3, 5.77.

7. The substantially pure X sex-associated membrane protein according to claim 2.

8. The substantially pure Y sex-associated membrane protein according to claim 2.