Process for effecting capillary electrophoresis

• US 5,348,658 A
• Filed: 02/17/1993
• Issued: 09/20/1994
• Est. Priority Date: 05/29/1990
• Status: Expired due to Term

• Current Assignee: Waters Technologies Corporation (Waters Corporation)
• Original Assignee: Waters Investments Limited (Waters Corporation)
• Inventors: Fuchs, Martin, Merion, Michael
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US08/018,647
• Time in Patent Office: 580 Days
• Field of Search: 204/180.1, 204/182.8, 204/299 R, 210/635, 210/656, 210/198.2, 210/748
• US Class Current: 210/656
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 30/6004 end pieces
• CPC Class Codes: G01N 30/6052 body

Abstract

An apparatus is provided for concentrating a solute sample which can be subsequently released for analysis by capillary electrophoresis. The apparatus comprises a tube containing a short length of packed particles or gel adapted to retain sample solutes which particles are retained in the tube by tube constrictions or porous plugs. The tube includes an inlet for introducing a sample into the tube and an outlet for introducing the retained sample into a capillary electrophoresis tube.

Claims

1. The process for effecting capillary separation in the apparatus which comprises a first capillary tube section comprising a capillary tube having an inner diameter between about 1 and 500 micrometers and containing a packing capable of retaining a solute sample from a first solvent and subsequently releasing said sample into a second solvent, said first tube section including means for retaining said packing within said first tube section while permitting fluid flow through said first tube section, said first tube section having an inlet and an outlet, said outlet being in fluid communication with a second capillary tube section, said second capillary tube section, having an inner diameter between about 1 and 500 micrometers and free of a packing material, and means for effecting said capillary separation process in said second capillary tube section, which comprises introducing a solution comprising a first solvent and a solute sample into said first tube section to effect retention of said solute sample by said packing while permitting said first solvent to pass through said first tube section, passing a second solvent through said first tube section to release said solute sample from said particulate packing and into said second capillary tube section and analyzing said solute sample in said second capillary tube section by a capillary separation process selected from the group consisting of capillary electrophoresis and micellar electrokinetic capillary chromatography.

2. The process of claim 1 wherein an electrolyte is introduced into said capillary tube section after said solute sample is introduced into said first tube section and prior to introducing said second solvent into said first tube section.

3. The process of claim 1 wherein said capillary separation process is capillary electrophoresis.

4. The process of claim 1 wherein said capillary separation process is micellar electrokinetic capillary chromatography.