Cucumber mosaic virus coat protein gene

• US 5,349,128 A
• Filed: 01/28/1993
• Issued: 09/20/1994
• Est. Priority Date: 08/19/1988
• Status: Expired due to Term

• Current Assignee: Seminis Vegetable Seeds Incorporated (Bayer AG)
• Original Assignee: The Upjohn Company (Pharmacia Corp.)
• Inventors: Kearney, Chris, Gonsalves, Dennis, Slightom, Jerry L., Quemada, Hector D.
• Primary Examiner(s): Fox, David T.
• Application Number:
  US08/010,423
• Time in Patent Office: 600 Days
• Field of Search: 536/23.72, 435/172.3, 435/252.3, 435/240.4, 435/320.1, 435/70.1, 800/205, 800/DIG. 18, 800/DIG. 40
• US Class Current: 800/301
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 2770/14022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The coat protein gene of cucumber mosaic virus strain WL (CMV-WL), the method of preparing it, its use to prepare transgenic plants and transgenic plants containing it are provided.

Claims

1. The coat protein gene from the WL strain of cucumber mosaic virus and having the sequence shown in Chart 1.

2. A plant transformation vector comprising the coat protein gene according to claim 1, the 35S promoter of califlower mosaic virus and the polyadenylation signal of the califlower mosaic 35S gene, wherein said promoter is upstream from said CMV-WL coat protein gene 5'"'"' untranslated region;

and said CMV-WL coat protein gene 5'"'"' untranslated region is upstream from a link to said polyadenylation signal of the califlower mosaic 35S gene.

3. A bacterial cell containing the plant transformation vector of claim 2.

4. A transformed plant cell containing the plant

5. A plant selected from the family cucurbitaceae comprising the transformed plant cell of claim 4.

6. A plant selected from the family solanaceae comprising the transformed plant cell of claim 4.