Method for reviving an electrode of a biosensor

• US 5,352,349 A
• Filed: 01/07/1993
• Issued: 10/04/1994
• Est. Priority Date: 08/30/1989
• Status: Expired due to Fees

• Current Assignee: Sankyo Company Limited (Daiichi Sankyo Company Limited)
• Original Assignee: Daikin Industries Ltd
• Inventors: Inamoto, Tomoyuki, Fujimura, Hidetaka, Nagata, deceased, Yasuhiro
• Primary Examiner(s): TUNG, TA HSUNG
• Application Number:
  US08/001,197
• Time in Patent Office: 635 Days
• Field of Search: 204/153.12, 204/153.17, 204/402, 204/403, 204/415
• US Class Current: 205/778
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/38 Cleaning of electrodes

Abstract

After a reverse bias is applied between a working electrode and a counter electrode or a reference electrode, a forward bias higher than a forward measurement bias is applied between the working electrode and the counter electrode or the reference electrode, before the forward measurement bias is applied. This reduces the time period before which an actual measuring operation is possible. When the bias is applied between the working electrode and the counter electrode or reference electrode, the bias is gradually increased in absolute value according to a predetermined time constant. This reduces damage to the membrane or membranes fixed on a surface of the electrode unit.

Claims

1. A method for reviving an electrode unit of a biosensor device which includes a working electrode and a counter electrode, and which measures a concentration of an object substance based upon an electrical signal generated between the working electrode and the counter electrode when a predetermined forward measurement bias voltage is applied to the working electrode on which a physiologically active substance is placed, the electrical signal being generated based upon a biological reaction of the object substance, said method comprising the steps of:

(1) applying a predetermined reverse bias voltage to the working electrode for a predetermined time period, and

(2) applying a predetermined forward measurement bias voltage to the working electrode,

wherein at least one of the bias voltages is gradually increased in absolute value according to a time constant to reach a predetermined value.

2. A method as set forth in claim 1, wherein the predetermined reverse bias voltage is gradually increased according to a first time constant until the absolute value thereof reaches a predetermined value, and the predetermined forward measurement bias voltage is gradually increased according to a second time constant until the absolute value thereof reaches a predetermined value.

3. A method as set forth in claim 2, wherein the second time constant is equal to the first time constant.

4. A method as set forth in claim 2, wherein the second time constant is longer than the first time constant.

5. A method for reviving an electrode unit of a biosensor device which includes a working electrode and a counter electrode, and which measures a concentration of an object substance based upon an electrical signal generated between the working electrode and the counter electrode when a predetermined forward measurement bias voltage is applied to the working electrode on which a physiologically active substance is placed, the electrical signal being generated based upon a biological reaction of the object substance, said method comprising the steps of:

(1) applying a predetermined reverse bias voltage to the working electrode for a first predetermined time period, and thereafter

(2) applying a forward bias voltage which is higher than the forward measurement bias voltage to the working electrode for a second predetermined time period before the forward measurement bias voltage is applied to the working electrode,

wherein at least one of the reverse bias voltage, the forward bias voltage, and the forward measurement bias voltage is gradually increased in absolute value according to a time constant to reach a predetermined value.

6. A method as set forth in claim 5, wherein the predetermined reverse bias voltage is gradually increased according to a first time constant until the absolute value thereof reaches a predetermined value, and the forward bias voltage is gradually increased according to a second time constant until the absolute value thereof reaches a predetermined value which is higher than the forward measurement bias voltage.

7. A method as set forth in claim 6, wherein the second time constant is equal to the first time constant.

8. A method as set forth in claim 6, wherein the second time constant is longer than the first time constant.