Holographic based bio-assay
First Claim
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1. An apparatus for detecting a target reactant, said apparatus comprising:
- a support structure;
a coherent light source;
a second reactant immobilized on said support structure at selected locations thereon, said locations corresponding to a predetermined pattern, said immobilized reactant binding said target reactant when said target and immobilized reactants are brought into contact with one another, wherein said predetermined pattern causes a holographic image to be generated at a predetermined image location when said immobilized reactant is bound to said target reactant and said bound reactants are illuminated by said coherent light source; and
detection means for detecting said holographic image.
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Abstract
An apparatus detects a target reactant that binds to an immobilized reactant. The apparatus generates a holographic image at a predetermined location when the reactants are present and bound to one another. The immobilized reactant is bound to a support surface at selected locations. The locations are chosen such that a holographic plate is generated when the target reactant binds to the immobilized reactant. An associated method may be used to detect antibody-antigen reactions, the binding of two strands of nucleic acid, the binding of an enzyme to one of its substrates, and so on.
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Citations
19 Claims
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1. An apparatus for detecting a target reactant, said apparatus comprising:
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a support structure; a coherent light source; a second reactant immobilized on said support structure at selected locations thereon, said locations corresponding to a predetermined pattern, said immobilized reactant binding said target reactant when said target and immobilized reactants are brought into contact with one another, wherein said predetermined pattern causes a holographic image to be generated at a predetermined image location when said immobilized reactant is bound to said target reactant and said bound reactants are illuminated by said coherent light source; and detection means for detecting said holographic image. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for detecting a target reactant, said method comprising the steps of:
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bringing said target reactant into contact with an immobilized reactant that is immobilized on a support structure at selected locations thereon, said locations corresponding to a predetermined pattern, said immobilized reactant binding said target reactant when said target and immobilized reactants are brought into contact with one another, wherein said predetermined pattern causes a holographic image to be generated at a predetermined image location when said immobilized reactant is bound to said target reactant and said bound reactants are exposed to coherent light; illuminating said support structure with a coherent light source; and measuring the intensity of light at said image location. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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Specification