Method and apparatus for optical measurement of bilirubin in tissue

• US 5,353,790 A
• Filed: 01/17/1992
• Issued: 10/11/1994
• Est. Priority Date: 01/17/1992
• Status: Expired due to Term

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Saidi, Iyad, Oelberg, David G., Jacques, Steven L.
• Primary Examiner(s): ZELE, KRISTA MICHELLE
• Application Number:
  US07/822,461
• Time in Patent Office: 998 Days
• Field of Search: 128/633, 128/664, 128/665, 128/653.1, 606/3, 356/39-41, 250/574, 250/226, 250/228
• US Class Current: 600/315
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61N 5/0621 Hyperbilirubinemia, jaundice treatment
• CPC Class Codes: G01N 21/4738 Diffuse reflection precedence is given to G01N21/55 - G01N21/57 if specular component is taken into consideration, e.g. also for testing fluids, fibrous materials
• CPC Class Codes: G01N 2201/065 Integrating spheres

Abstract

A method and apparatus for the determination of bilirubin concentration in tissue such as skin, particularly neonatal skin. Light reflected from the skin under test is analyzed to determine bilirubin concentration in the skin, corrected for maturity-dependent optical properties of the skin, the amount of melanin in the skin and the amount of blood in the skin. Reflected red to infrared light is used to determine maturity-dependent optical properties, reflected red light is used to determine melanin content, and reflected yellow-orange light is used to determine the amount of blood in the skin. Then, these quantities are used, in combination with reflected blue light, to calculate cutaneous bilirubin concentration.

Claims

1. A method for the transcutaneous determination of bilirubin concentration in tissue, comprising:

illuminating said tissue with light;

detecting a frequency spectrum of light reflected from said tissue;

calculating, from a first portion of said spectrum, a first parameter indicative of a maturity of said tissue;

calculating, from a second portion of said spectrum, a second parameter indicative of an amount of melanin in said tissue;

calculating, form a third portion of said spectrum, a third parameter indicative of a blood content of said tissue;

calculating, from a fourth portion of said spectrum, a fourth parameter indicative of an uncorrected bilirubin concentration in said tissue; and

calculating a connected bilirubin concentration in said tissue as a function of said first, second, third and fourth parameters.

2. The method of claim 1, wherein said first portion of said spectrum is red to infrared light.

3. The method of claim 2, said red to infrared light having wavelengths in the range of 650 nm to 800 nm.

4. The method of claim 1, wherein said second portion of said spectrum is red light.

5. The method of claim 4, said red light having a wavelength of approximately 650 nm.

6. The method of claim 1, wherein said third portion of said spectrum is yellow-orange light.

7. The method of claim of 6, said yellow-orange light having a wavelength of approximately 585 nm.

8. The method of claim 1, wherein said fourth portion of said spectrum is blue light.

9. The method of claim 8, wherein said blue light has a wavelength of approximately 460 nm.

10. The method of claim 1, further comprising:

calculating, from a fifth portion of said spectrum, a fifth parameter indicative of a depth of blood within said tissue; and

calculating a concentration of bilirubin in said tissue as a function of said first, second, third, fourth and fifth parameters.

11. The method of claim 10, wherein said fifth portion of said spectrum is purple-blue light.

12. The method of claim 11, wherein said purple-blue light has a wavelength of approximately 420 nm.

13. An apparatus for detecting a concentration of bilirubin in tissue, comprising:

a light source adapted to direct light onto tissue under test;

a light detector, adapted to detect a spectrum of light reflected form said tissue; and

a computer means, connected to said light source and light detector, for calculating a first parameter indicative of a maturity of said tissue form a magnitude of a first portion of said spectrum, for calculating a second parameter indicative of an amount of melanin in said tissue from a second portion of said spectrum, for calculating a third parameter indicative of a blood content of said tissue from a third portion of said spectrum, for calculating a fourth parameter indicative of a raw bilirubin concentration form a fourth portion of said spectrum, and for calculating bilirubin concentration in said tissue as a function of said first, second, third and fourth parameters.