Analyte detection device including a hydrophobic barrier for improved fluid flow
First Claim
1. An immunochromatographic assay device for detection of an analyte comprising:
- a housing having an opening for introduction of a liquid sample;
first liquid permeable material in said housing under said opening to receive a liquid sample introduced through said opening and defining a sample receiving zone;
second liquid permeable material in said housing under said first liquid permeable material in fluid contact with said first liquid permeable material to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone;
third liquid permeable material in said housing adjacent said first liquid permeable material and in fluid contact with said second permeable material to receive sample from said second liquid permeable material, said third liquid permeable located on top of said second liquid permeable material;
a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material;
an elongated sheet of wicking material in fluid contact with said third liquid permeable material in fluid contact with said third liquid permeable material to receive sample therefrom, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte; and
a detecting reagent located in one of said liquid permeable materials to detect the presence of bound analyte in said elongated sheet of wicking material.
2 Assignments
0 Petitions
Accused Products
Abstract
An immunochromatographic assay device for the detection of an analyte in a liquid sample, the device including a housing that contains an opening for the application of the liquid sample and multiple liquid permeable materials located in the housing that are adapted to receive, treat and facilitate the movement of the sample through the housing; preferably, a first liquid permeable material is adapted to receive the liquid sample; a second liquid permeable material is positioned under the first liquid permeable material; a third liquid permeable material is positioned above the second liquid material; means are provided to prevent liquid communication between the first and third liquid permeable materials; a wicking material, in fluid contact with the third liquid permeable material receives the sample; and reagents are positioned in the housing that display the results of the immunoassay.
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Citations
25 Claims
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1. An immunochromatographic assay device for detection of an analyte comprising:
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a housing having an opening for introduction of a liquid sample; first liquid permeable material in said housing under said opening to receive a liquid sample introduced through said opening and defining a sample receiving zone; second liquid permeable material in said housing under said first liquid permeable material in fluid contact with said first liquid permeable material to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone; third liquid permeable material in said housing adjacent said first liquid permeable material and in fluid contact with said second permeable material to receive sample from said second liquid permeable material, said third liquid permeable located on top of said second liquid permeable material; a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material; an elongated sheet of wicking material in fluid contact with said third liquid permeable material in fluid contact with said third liquid permeable material to receive sample therefrom, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte; and a detecting reagent located in one of said liquid permeable materials to detect the presence of bound analyte in said elongated sheet of wicking material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 12)
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- 10. An immunocromatographic assay device for detection of an analyte in a liquid sample comprising a housing, a sample receiving zone in said housing, an elongated sheet of wicking material in fluid communication with said sample receiving zone, wherein said elongated sheet of wicking material supports an immobilized capture molecule for said analyte, and a detecting reagent located in said sample receiving zone to detect the presence of bound analyte in said elongated sheet of wicking material, said sample receiving zone comprising a sample treatment pad having a first liquid permeable material for receiving the liquid sample, a second liquid permeable material in fluid communication with said first liquid permeable material in order to receive sample from said first liquid permeable material, and a third liquid permeable material in fluid communication with said second liquid permeable material and adjacent said first liquid permeable material, wherein a barrier comprising a hydrophobic material is interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material.
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21. A sample treatment pad for use in an immunochromatographic assay device comprising at least
a first liquid permeable material; -
a second liquid permeable material in fluid contact with said first liquid permeable material in order to receive sample from said first liquid permeable material, said second liquid permeable material being absorbent and defining a sample transport zone; a third liquid permeable material adjacent said first liquid permeable material and in fluid contact with said second liquid permeable material in order to receive sample from said second liquid permeable material; and a barrier comprising a hydrophobic material interposed between said first and third liquid permeable materials to prevent direct transfer of sample from said first liquid permeable material to said third liquid permeable material. - View Dependent Claims (22, 23, 24, 25)
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Specification