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Method and apparatus for quantitative fluorescence measurements

  • US 5,355,215 A
  • Filed: 09/30/1992
  • Issued: 10/11/1994
  • Est. Priority Date: 09/30/1992
  • Status: Expired due to Term
First Claim
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1. A method for reducing background fluorescence during the simultaneous measurement of a fluorescent emission from each of a plurality of samples contained in a multiple well plate, each sample being disposed adjacent a background fluorescence producing medium, said method including the steps of:

  • providing a light source which emits illumination a first wavelength selected to excite the fluorescent emission of light of a second wavelength by said samples;

    disposing said light source so as to project illumination of said first wavelength onto and through a portion of a bottom surface of each of said wells at a first angle of incidence, so as to penetrate said samples and excite the fluorescent emission of light of said second wavelength therefrom through the bottom surfaces of said wells;

    disposing an optical sensor, responsive to illumination of said second wavelength, so as to receive light emitted from the bottom surface of each of said wells at a second angle wherein at least one of said first or second angles is oblique to said bottom surface so as to increase the extent to which the paths associated with said illumination and said sensing intersect within said sample as compared to said background fluorescence producing medium; and

    sensing the light emitted from the bottom surface of each of said wells with said optical sensor.

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