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Determination of substrate of coenzyme requiring enzyme

  • US 5,356,780 A
  • Filed: 03/17/1993
  • Issued: 10/18/1994
  • Est. Priority Date: 10/15/1990
  • Status: Expired due to Term
First Claim
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1. A method of assaying within a liquid sample a substrate for a coenzyme-requiring enzyme which method comprises contacting the sample with an assay system including (i) a substrate analogue and (ii) a moiety which specifically binds the substrate without catalyzing the conversion of the substrate;

  • the substrate analogue being a substance which competes with the substrate for binding to the moiety, and determining the presence or amount of the substrate by monitoring the extent to which there are formed or remain complexes between the moiety and at least one of the substrate analogue and the substrate;

    wherein the moiety is selected from the group consisting of an apoenzyme which is not bound to its functional coenzyme, a partly denatured enzyme, a deactivated enzyme, a genetically engineered deactivated enzyme and a deactivated artificial enzyme.

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