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Analytical test apparatus with on board negative and positive control

  • US 5,356,782 A
  • Filed: 09/03/1992
  • Issued: 10/18/1994
  • Est. Priority Date: 09/03/1992
  • Status: Expired due to Term
First Claim
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1. An apparatus for determining an analyte of interest in a liquid sample comprising an absorptive material and having a plurality of zones in the direction of fluid flow, wherein:

  • (i) a first negative control zone of said absorptive material contains an immobilized reactant which does not bind to said analyte of interest;

    (ii) a second analytical zone of said absorptive material contains an immobilized reactant which specifically binds to said analyte of interest; and

    (iii) a third positive control zone of said absorptive material contains both (a) an immobilized reactant which specifically binds to said analyte of interest, and (b) a portion of the analyte of interest in solubilizible form, wherein said portion of the analyte is solubilized when contacted with the liquid sample and reacts with the immobilized reactant of said third zone.

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