Interferant eliminating biosensor

• US 5,356,786 A
• Filed: 12/02/1993
• Issued: 10/18/1994
• Est. Priority Date: 03/04/1991
• Status: Expired due to Term

• Current Assignee: Therasense Incorporated (Abbott Laboratories)
• Original Assignee: E. Heller & Company
• Inventors: Maidan, Ruben, Heller, Adam
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): Leary, Louise N.
• Application Number:
  US08/161,682
• Time in Patent Office: 320 Days
• Field of Search: 435/14, 435/28, 435/26, 435/24, 435/288, 435/291, 435/817, 204/403, 204/157.15, 204/153.1, 429/111, 429/40, 373/80
• US Class Current: 205/778
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Interferant eliminating analyte sensors and sensing process prevent erroneous assays. Glucose electrodes were coated with an oxidizing enzyme (peroxidase) which allows hydrogen peroxide to selectively oxidize ascorbate, urate, bilirubin, and acetaminophenol in the presence of glucose. Hydrogen peroxide may be added to the assayed solution or generated in situ. The oxidizing enzyme was prevented from causing undesired reduction currents at the glucose electrode by preventing contact of the oxidizing enzyme with the glucose electrode, or by increasing the applied voltage.

Claims

1. A biosensor comprising:

an electrode on which an analyte is electrooxidized at a given applied potential; and

an interferant-eliminating layer substantially covering the electrode but electrically isolated therefrom at the given applied potential,

said interferant-eliminating layer comprising a catalyst which is capable of catalizing substantial oxidation of a plurality of interferants but not substantial oxidation of the analyte,

wherein the catalyst mediates the following reaction;

##STR4## where AH₂ represents an interfering compound and A represents a non-interfering compound.

2. The biosensor of claim 1, wherein the catalyst is a natural or synthetic enzyme.

3. The biosensor of claim 2, wherein the enzyme is peroxidase.

4. The biosensor of claim 3, wherein the peroxidase is horseradish peroxidase, cytochrome c peroxidase, chloroperoxidase, lactoperoxidase, thyroid peroxidase, Japanese horseradish peroxidase a, Japanese horseradish peroxidase c, myeloperoxidase, NADH peroxidase, turnip peroxidase A₁, turnip peroxidase B, turnip peroxidase D, or glutathione peroxidase.

5. The biosensor of claim 2, wherein the catalyst is an enzyme having iron (III) porphyrins.

6. The biosensor of claim 1, wherein an insulating barrier is positioned between said catalyst and said electrode.

7. The biosensor of claim 6, wherein said insulating barrier is a membrane.

8. The biosensor of claim 7, wherein said membrane is cellulose acetate, polycarbonate, or perfluorinated membrane.

9. The biosensor of claim 1, wherein said catalyst is immobilized within a polymeric matrix.

10. The biosensor of claim 1, wherein said electrode comprises an enzyme mediating oxidation of said analyte.

11. The biosensor of claim 10, wherein said enzyme is an oxidoreductase.

12. The biosensor of claim 11, wherein said oxidoreductase is glucose oxidase, lactate oxidase, xanthene oxidase, cholesterol oxidase, pyruvate oxidase, L-amino acid oxidase, D-amino acid oxidase, alcohol oxidase, urease, urate oxidase, aldehyde oxidase, glycollate oxidase, or sarcosine oxidase.

13. The biosensor of claim 12, wherein the oxidoreductase is glucose oxidase.

14. A process for analyzing an analyte in the presence of interferants in a sample comprising the steps of:

contacting a sample with an interferant eliminating biosensor having an electrode and an interferant eliminating catalyst;

substantially oxidizing interferants in said sample by an oxidation reaction catalyzed by the interferant eliminating catalyst; and

detecting the analyte at the electrode in the absence of substantial interference.

15. The process of claim 14, wherein said catalyst mediates the following reaction:

##STR5## where AH₂ represents an interfering compound and A represents a non-interfering compound.