Oxygen electrode

• US 5,358,619 A
• Filed: 12/17/1992
• Issued: 10/25/1994
• Est. Priority Date: 09/17/1990
• Status: Expired due to Fees

• Current Assignee: Fujitsu Limited
• Original Assignee: Fujitsu Limited
• Inventors: Suzuki, Hiroaki, Sugama, Akio, Kojima, Naomi
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US07/993,486
• Time in Patent Office: 677 Days
• Field of Search: 204/415, 204/418
• US Class Current: 204/403.06
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/404 Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid , e.g. Clark-type oxygen sensors
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A small oxygen electrode and a method of bonding a fluorine resin film are disclosed. This small oxygen electrode includes a flat electrode substrate having at least two electrodes (a working electrode and a counter electrode) formed thereon, and a container substrate having dents formed to confront the two electrodes and contain an electrolyte therein. The container substrate which is bonded to the flat electrode substrate, so that the dent confronting the electrode constituting the working electrode has a through hole extending to the side opposite to the flat electrode substrate and a gas-permeable film is formed to cover the through hole. By using this dent structure, the preparation of an oxygen electrode can be conducted while maintaining a wafer form throughout the process, and formation of an electrode pattern can be facilitated. The method of bonding the fluorine resin film includes treating the fluorine resin film with an agent containing metallic sodium, then treating the fluorine resin film with a silane coupling agent. The treated fluorine resin film is fusion bonded by heating, and if necessary, conducting subsequent treatments in vacuo. By adopting this method, the fluorine resin film can be bonded tightly to the substrate and peeling of the fluorine resin film from the substrate can be prevented.

Claims

1. An oxygen electrode comprising:

a flat electrode substrate having at least a working electrode and a counter electrode formed thereon

a container substrate having dents formed to confront said working electrode and said counter electrode and having fine grooves for connecting said dents to one another said dents and said fine grooves containing an electrolyte containing water therein, said container substrate being bonded to said flat electrode substrate, wherein of said dents, said dent confronting said working electrode has a through hole extending to a side opposite to said flat electrode substrate; and

a gas-permeable film which is impermeable to said electrolyte containing water, is formed to cover said through hole.

2. An oxygen electrode as set forth in claim 1, further comprising:

a reference electrode formed on said flat electrode substrate, and

an electrolyte-injecting dent formed on said container substrate to confront said reference electrode.

3. An oxygen electrode as set forth in claim 2, wherein said working electrode and said counter electrode formed on said flat electrode substrate are separated from one another by such a distance that said working and counter electrodes are not influenced by products formed on their respective surfaces.

4. An oxygen electrode as set forth in claim 2, further comprising an additional elongated groove for connecting said electrolyte injecting dent to said other dents.

5. An oxygen electrode as set forth in claim 2, wherein each of said dents is formed on the peripheral edge portion of the through hole on the surface, opposite to the bonded surface, of said container substrate, and each of said dents is covered with said gas-permeable film.

6. An oxygen electrode as set forth in claim 2, wherein said flat electrode substrate is selected from the group consisting of a borosilicate glass substrate, a lead glass substrate, a silicon substrate having, formed on the surface thereof, a film of a borosilicate glass, a silicon substrate having, formed on the surface thereof, a film of a lead glass, a glass substrate having, formed on the surface thereof, a film of a borosilicate glass containing a Pyrex glass, a glass substrate having, formed on the surface thereof, a film of a lead glass, and a silicon substrate having, formed on the surface thereof, a thermal oxidation film.

7. An oxygen electrode as set forth in claim 2, wherein said working and counter electrodes are formed in a shallow groove formed on said flat electrode substrate by etching in accordance with an electrode pattern of said working and counter electrodes.

8. An oxygen electrode as set forth in claim 2, wherein said container substrate is a (100) plane silicon substrate.

9. An oxygen electrode as set forth in claim 2, further comprising a pad formed at one end of each of said working and counter electrodes, wherein said pad has a size such that a socket terminal of an integrated circuit (IC) is directly gripped in said pad.

10. An oxygen electrode as set forth in claim 1, wherein said working electrode and said counter electrode formed on said flat electrode substrate are separated from one another by such a distance that said working and counter electrodes are not influenced by products formed on their respective surfaces.

11. An oxygen electrode as set forth in claim 1, wherein each of said dents is formed on the peripheral edge portion of the through hole on the surface, opposite to the bonded surface, of said container substrate, and each of said dents is covered with said gas-permeable film.

12. An oxygen electrode as set forth in claim 1, wherein said flat electrode substrate is selected from the group consisting of a borosilicate glass substrate, a lead glass substrate, a silicon substrate having, formed on the surface thereof, a film of a borosilicate glass, a silicon substrate having, formed on the surface thereof, a film of a lead glass, a glass substrate having, formed on the surface thereof, a film of a borosilicate glass containing a Pyrex glass, a glass substrate having, formed on the surface thereof, a film of a lead glass, and a silicon substrate having, formed on the surface thereof, a thermal oxidation film.

13. An oxygen electrode as set forth in claim 1, wherein said working and counter electrodes are formed in a shallow groove formed on said flat electrode substrate by etching in accordance with an electrode pattern of said working and counter electrodes.

14. An oxygen electrode as set forth in claim 1, wherein said container substrate is a (100) plane silicon substrate.

15. An oxygen electrode as set forth in claim 1, wherein said gas-permeable film is a fluorinated ethylene propylene (FEP) film.

16. An oxygen electrode as set forth in claim 1, further comprising a pad formed at one end of each of said working and counter electrodes, wherein said pad has a size such that a socket terminal of an integrated circuit (IC) is directly gripped in said pad.

17. An oxygen electrode comprising:

an electrode substrate having first and second electrodes formed thereon;

a container substrate having dents which confront said first and second electrodes and an elongated grove for connecting the dents to one another, said dents and said elongated groove containing an electrolyte containing water therein, said container substrate being bonded to said electrode substrate so that said dent confronting said first electrode has a through hole extending to a side opposite said electrode substrate; and

a gas-permeable film which is impermeable to said electrolyte containing water, covering said through hole.

18. An oxygen electrode comprising:

a dent covered with an insulating film formed on one of the surfaces of a substrate;

a plurality of electrodes formed in a mutually spaced-apart relation at a bottom portion of said dent;

an electrolyte filled in said dent; and

a gas-permeable film formed at portions where said electrodes are formed, other than a through-hole for injecting said electrolyte, in such a manner as to cover said dent.

19. An oxygen electrode according to claim 18, wherein said substrate on which said dent is to be formed is a silicon substrate or a glass substrate.

20. An oxygen electrode according to claim 18, which includes a reference electrode in addition to a working electrode and a counter electrode.

21. An oxygen electrode according to claim 18, wherein said gas-permeable film is a film of fluorinated ethylene propylene resin.

22. A biosensor comprising:

a dent covered with an insulating film formed on one of the surfaces of a substrate;

a plurality of electrodes formed in a mutually spaced-part relation at a bottom portion of said dent;

an electrolyte filled in said dent;

a gas-permeable film formed at portions where said electrodes are formed, other than at a through-hole for injecting said electrolyte, in such a manner as to cover said dent; and

at least one layer of an enzyme- or microorganism- immobilizing film formed on said gas-permeable film.

23. A biosensor comprising:

a dent covered with an insulating film formed on one of the surfaces of a substrate;

a plurality of electrodes formed in a mutually spaced-part relation at a bottom portion of said dent;

an electrolyte filled in said dent;

a gas-permeable film formed at portions where said electrodes are formed, other than a through-hole for injecting said electrolyte, in such a manner as to cover said dent; and

an enzyme or a microorganism-immobilizing film formed between said dent and said gas-permeable film.

24. A biosensor comprising:

a dent covered with an insulating film formed on one of the surfaces of a substrate;

a plurality of electrodes formed in a mutually spaced-part relation at a bottom portion of said dent;

an electrolyte filled in said dent;

a gas-permeable film formed at portions where said electrodes are formed, other than at a through-hole for injecting said electrolyte, in such a manner as to cover said dent;

a first enzyme- or microorganism-immobilizing film formed between said dent and said gas-permeable film; and

a second enzyme- or microorganism-immobilizing film formed on said gas-permeable film.