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DNA sequencing

  • US 5,360,523 A
  • Filed: 02/17/1993
  • Issued: 11/01/1994
  • Est. Priority Date: 03/29/1984
  • Status: Expired due to Fees
First Claim
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1. A method for sequencing DNA comprising:

  • applying fluorescently marked DNA samples at a plurality of locations for electrophoresing in a plurality of channels through a gel electrophoresis slab;

    establishing electrical potential across said gel electrophoresis slab wherein DNA samples are resolved in accordance with the size of DNA fragments in said gel electrophoresis slab into fluorescently marked DNA bands;

    moving a scanning means with a carriage assembly a direction substantially perpendicular to a direction of the plurality of channels; and

    scanning the separated samples photoelectrically with a laser and a sensor mounted to the carriage assembly, wherein the laser scans with scanning light ata scanning light frequency within an absorbent spectrum of said fluorescently marked DNA samples and sensing light at an emission frequency of the marked DNA;

    said step of scanning including the substeps of focusing a microscope on a gel portion of an electrophoresis slab at least at two different locations along a path of a single scan.

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