Method and apparatus for dynamic correction of microscopic image signals

• US 5,361,140 A
• Filed: 02/18/1992
• Issued: 11/01/1994
• Est. Priority Date: 02/18/1992
• Status: Expired due to Term

• Current Assignee: Tripath Imaging Incorporated (Becton, Dickinson & Co)
• Original Assignee: NeoPath, Inc. (Becton, Dickinson & Co)
• Inventors: Hayenga, Jon W., Schmidt, Robert C.
• Primary Examiner(s): Coles, Jr., Edward L.
• Assistant Examiner(s): LEE, CHEUKFAN
• Application Number:
  US07/838,065
• Time in Patent Office: 987 Days
• Field of Search: 358/445, 358/475, 358/446, 358/461, 358/444, 358/480, 358/482, 358/483, 358/406, 358/487, 358/447, 358/213.18, 358/213.19, 358/228, 358/211, 358/113, 250/208.1, 348/255, 348/257, 348/229, 348/230, 348/241
• US Class Current: 358/446
• CPC Class Codes: H04N 1/401 Compensating positionally unequal response of the pick-up or reproducing head H04N1/403 takes precedence

Abstract

A circuit is provided for correcting an image signal received from a camera wherein the camera includes a plurality of charge coupled devices, each for providing a light signal indicative of the intensity of light incident thereon and wherein the plurality of light signals from the plurality of charge coupled devices are indicative of an image received by the camera. The correction circuit includes a gain memory for storing a plurality of gain correction factors wherein each gain correction factor is associated with a respective charge coupled device of the camera. The correction circuit further includes an offset memory for storing a plurality of offset correction factors wherein each offset correction factor is associated with a respective one of the plurality of charged coupled devices. The gain memory is responsive to a control circuit for multiplying the plurality of electric signals by the appropriate gain correction factor. Similarly, the offset memory is responsive to a control circuit for adding the appropriate one of the plurality of offset correction factors with the signal from its respective charge coupled device. A light detection circuit is also provided for normalizing the corrected plurality of signal to make the resulting measurement a measurement of the percentage of transmission of the focused slide specimen.

Claims

1. Apparatus for dynamically correcting an image signal comprising:

camera means for providing an image signal, wherein the image signal represents a tranmissivity of an object;

light means for providing light to illuminate the object and for providing a light signal indicative of the intensity of said light; and

data processing means responsive to said light signal for dynamically correcting said image signal so that said image signal is corrected for variations in the intensity of said light.

2. The apparatus as recited in claim 1 wherein said data processing means comprises an analog-to-digital converter having an analog input for receiving the image signals and a reference input for receiving said light signal, said analog-to-digital converter having an output providing the corrected image signals.

3. The apparatus as recited in claim 1 wherein the camera includes a plurality of charge coupled devices for providing a respective plurality of pixel signals and wherein the plurality of pixel signals combine to provide the image signal, said data processing means comprising:

offset correction means for storing a plurality of offset correction factors, each associated with a respective one of said plurality of charge coupled devices, said offset correction means being further constructed to combine said plurality of offset correction factors with the respective ones of the plurality of pixel signals to thereby correct the image signal for variation in offsets between the plurality of charge coupled devices.

4. The apparatus as recited in claim 3 wherein said offset correction means further comprises means responsive to variations in the intensity of said light signal for varying the value of said plurality of offset correction factors as the image signal is provided by the camera.

5. The apparatus as recited in claim 3 wherein said offset correction means further comprises:

memory means for storing said plurality of offset correction factors; and

a digital-to-analog converter having a digital input for receiving the plurality of offset correction factors and a reference input for receiving said light signal, said digital-to-analog converter having an output providing the plurality of offset correction factors in response to the light signal.

6. The apparatus as recited in claim 1 wherein the camera includes a plurality of charge coupled devices for providing a respective plurality of pixel signals wherein the plurality of pixel signals combine to provide the image signal, said data processing means comprises:

gain correction means for storing a plurality of gain factors, each associated with a respective one of said plurality of charge coupled devices, said gain correction means being further constructed to multiply said plurality of gain factors by the respective ones of the plurality of pixel signals to thereby correct the image signal for variations in the sensitivity and incident illumination of the plurality of charge coupled devices.

7. The apparatus as recited in claim 6, further comprising:

offset correction means for storing a plurality of offset correction factors, each associated with a respective one of said plurality of charge coupled devices, said offset correction means being further constructed to add said plurality of offset correction factors to the respective ones of the plurality of pixel signals to thereby correct the image signal for variation in offsets between the plurality of charge coupled devices.

8. The apparatus as recited in claim 7 wherein said offset correction means further comprises means responsive to the variations in the intensity of said light signal for varying the value of said plurality of offset correction factors as the image signal is provided by the camera.

9. The apparatus as recited in claim 7 wherein said offset correction means further comprises:

memory means for storing said plurality of offset correction factors; and

a digital-to-analog converter having a digital input for receiving the plurality of offset correction factors and a reference input for receiving said light signal, said digital to analog converter having an output which provides the plurality of offset correction factors in response to the light signal.

10. A circuit for correcting an image signal comprising:

camera means for providing an image signal, wherein the image signal represents a transmissivity of an object and wherein the camera includes a plurality of charge coupled devices for providing a respective plurality of pixel signals wherein the plurality of pixel signals combine to provide the image signal;

memory means for storing a plurality of correction factors wherein each of said plurality of correction factors is associated with a respective one of the plurality of charge couples devices;

signal processing means for combining each of said plurality of correction factors with the respective one of the plurality of pixel signals with which it is associated; and

light means for providing light to illuminate the object and for providing a light signal indicative of the intensity of said light, said signal processing means being responsive to said light signal for altering said plurality of correction factors.

11. The circuit as recited in claim 10 wherein said signal processing means further comprises means for adding said plurality of correction factors to the respective ones of the plurality of pixel signals.

12. The circuit as recited in claim 10 wherein said signal processing means further comprises means for multiplying said plurality of correction factors to the respective ones of the plurality of pixel signals.

13. The circuit as recited in claim 10 wherein said memory means comprises means for storing a plurality of gain correction factors each associated with a respective one of the plurality of charge coupled devices and a plurality offset correction factors each associated with a respective one of the plurality of charge coupled devices.

14. The circuit as recited in claim 13 wherein said signal processing means comprises:

an adder for adding said plurality of offset correction factors to the respective ones of the plurality of pixel signals; and

a multiplier for multiplying the plurality of gain correction factors to the respective ones of the plurality of pixel signals.

15. An apparatus for dynamically correcting an image signal provided by a camera wherein the image signal represents a transmissivity of an object, said apparatus comprising:

light means for providing light to illuminate the object and for providing a light signal indicative of the intensity of said light; and

data processing means responsive to said light signal for dynamically correcting said image signal so that said image signal is corrected for variations in the intensity of said light wherein said data processing means comprises an analog-to-digital converter having an analog input for receiving the image signals and a reference input for receiving said light signal, said analog-to-digital converter having an output providing the corrected image signals.

16. The apparatus as recited in claim 15 wherein the camera includes a plurality of charge coupled devices for providing a respective plurality of pixel signals and wherein the plurality of pixel signals combine to provide the image signal, said data processing means comprising an offset correction means for storing a plurality of offset correction factors, each associated with a respective one of said plurality of charge coupled devices, said offset correction means being further constructed to combine said plurality of offset correction factors with the respective ones of the plurality of pixel signals to thereby correct the image signal for variation in offsets between the plurality of charge coupled devices.

17. The apparatus as recited in claim 16 wherein said offset correction means further comprises means responsive to variations in the intensity of said light signal for varying the value of said plurality of offset correction factors as the image signal is provided by the camera.

18. The apparatus as recited in claim 16 wherein said offset correction means further comprises:

memory means for storing said plurality of offset correction factors; and

a digital-to-analog converter having a digital input for receiving the plurality of offset correction factors and a reference input for receiving said light signal, said digital-to-analog converter having an output providing the plurality of offset correction factors in response to the light signal.

19. The apparatus as recited in claim 15 wherein the camera includes a plurality of charge coupled devices for providing a respective plurality of pixel signals wherein the plurality of pixel signals combine to provide the image signals and said data processing means comprises a gain correction means for storing a plurality of gain factors, each associated with a respected one of said plurality of charge coupled devices, said gain correction means being further constructed to multiply said plurality of gain factors by the respective ones of the plurality of pixel signals to thereby correct the image signals for variations in the sensitivity and incident illumination of the plurality of charge coupled devices.

20. The apparatus as recited in claim 19, further comprising offset correction means for storing a plurality of offset correction factors, each associated with a respective one of said plurality of charge coupled devices, said offset correction means being further constructed to add said plurality of offset correction factors to the respective ones of the plurality of pixel signals to thereby correct the image signal for variation in offsets between the plurality of charge coupled devices.

21. The apparatus as recited in claim 20 wherein said offset correction means further comprises means responsive to the variations in the intensity of said light signal for varying the value of said plurality of offset correction factors as the image signal is provided by the camera.

22. The apparatus as recited in claim 20 wherein said offset correction means further comprises:

memory means for storing said plurality of offset correction factors; and

a digital-to-analog converter having a digital input for receiving the plurality of offset correction factors and a reference input for receiving said light signal, said digital-to-analog converter having an output which provides the plurality of offset correction factors in response to the light signal.