Biochemical analyzer and attenuated total reflection prism cell used in said analyzer

• US 5,362,445 A
• Filed: 05/17/1993
• Issued: 11/08/1994
• Est. Priority Date: 07/27/1990
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Buhrer, Thomas, Fujii, Toshiko, Watanabe, Yoshio, Miyahara, Yuji
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Collins, Laura E.
• Application Number:
  US08/073,640
• Time in Patent Office: 540 Days
• Field of Search: 422/82.09, 422/82.11, 422/66, 422/67, 436/171, 436/178, 435/808, 356/36, 250/328, 250/304, 250/341
• US Class Current: 422/82.09
• CPC Class Codes: G01N 2021/0385 Diffusing membrane; Semipermeable membrane
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A biochemistry analyzer includes, an attenuated total reflection prism, a sample cell disposed on a surface of the prism, a sample supplier, a device for disposing more objective molecules in the liquid sample to a near portion of the sample which is near the surface of the prism, an infrared light source for generating an infrared light which is transmitted to the attenuated total reflection prism, a detector for detecting the infrared light which is reflected from the prism after the far portion of the sample is solidified, and a computer for calculating signals from the detector. As the objective molecules are gathered near the prism, accuracy of the biochemistry analyzer becomes higher.

Claims

1. A biochemistry analyzer for analyzing an objective substance in a liquid sample, comprising:

a housing including therein;

an attenuated total reflection prism;

a sample prism cell disposed on a side surface of the attenuated total reflection prism;

a sample supplying means for supplying a liquid sample to the sample prism cell;

distributing means in said sample prism cell for distributing an objective substance in the liquid sample to concentrate the objective substance at the side surface of the prism by solidifying the liquid sample in the sample prism cell with solidifying means external to the sample prism cell;

an infrared light source for transmitting infrared light through the objective substance to be reflected from the attenuated total reflection prism; and

a detector for detecting the infrared light which is reflected from the attenuated total reflection prism after the liquid sample is solidified, and for providing absorption spectrum data of the detected infrared light with respect to the objective substance and the liquid sample; and

a computer for receiving the absorption spectrum data from the detector and for calculating the detected absorption spectrum from the absorption spectrum data.

2. A biochemistry analyzer as defined in claim 1, wherein said solidifying means includes cooling means for solidifying the liquid sample from a fist portion of the liquid sample to a second portion of the liquid sample, said second portion of the liquid sample being in direct contact with said side surface of the attenuated total reflection prism so as to distribute more of said objective substance in the liquid sample toward said side surface of the attenuated total reflection prism.

3. A biochemistry analyzer as defined in claim 2, further comprising a double window on the cooling means which passes the infrared light from the infrared light source to the prism.

4. A biochemistry analyzer as defined in claim 2, further comprising a heating means for heating the sample prism cell after the detector detects the absorption spectrum of the liquid sample, and a washing means for washing the sample prism cell after the heating means heats the sample prism cell.

5. A biochemistry analyzer as defined in claim 2, further comprising a Dewar bottle for adding a cooling medium, disposed on or over the sample cell.

6. A biochemistry analyzer as defined in claim 1, wherein said distributing means includes a film for separating the objective substance from nonobjective substances by weight.

7. A biochemistry analyzer as defined in claim 6, wherein a region defined between said film and said prism contains distilled water.

8. A biochemistry analyzer as defined in claim 6, wherein said film is a porous film through which is passed the objective substance in order to prevent such passage of the nonobjective substances.

9. A biochemistry analyzer as defined in claim 6, wherein said film is a filter having small holes of diameter about 15 nm, said filter including an activated layer and a porous layer.

10. A biochemistry analyzer as defined in claim 1, wherein the distributing means includes separation means for separating the objective substance from nonobjective substances contained in the liquid sample.

11. A biochemistry analyzer for analyzing an objective substance in a liquid sample, comprising:

a housing including therein;

an attenuated total reflection prism;

a sample prism cell disposed on a side surface of the attenuated total reflection prism;

a sample supplying means for supplying a liquid sample to the sample prism cell;

distributing means for distributing an objective substance in the liquid sample in conjunction with a cooling means external to the sample prism cell for freezing the liquid sample progressively from a first portion of the liquid sample to a second portion of the liquid sample, said second portion of the liquid sample being in direct contact with said side surface of the attenuated total reflection prism so as to distribute more of the objective substance in the liquid sample toward the side surface of the attenuated total reflection prism;

an infrared light source for transmitting infrared light through the objective substance to be reflected from the attenuated total reflection prism; and

a detector for detecting the infrared light which is reflected from the attenuated total reflection prism after the second portion of the liquid sample is frozen, and for providing an output signal representative of the detected infrared light; and

a computer for receiving and analyzing the signal from the detector.

12. A biochemistry analyzer as defined in claim 11,

wherein said distributing means includes separation means, disposed in the sample prism cell, for separating the objective substance from nonobjective substances contained in the liquid sample.