Methods and containment system for storing, reconstituting, dispensing and harvesting cell culture media
First Claim
1. A containment system for use with cell culture media, the containment system comprising:
- a) a mixing bag having a mixing chamber formed therein;
b) a storage bag having a storage chamber formed therein, the storage bag substantially enclosing the mixing bag;
c) means for introducing cell culture media constituents into the mixing chamber;
d) means for conveying liquid cell culture media from the mixing chamber to the storage chamber; and
e) means for dispensing liquid cell culture media from the storage chamber.
3 Assignments
0 Petitions
Accused Products
Abstract
A cell culture media containment system is provided having a mixing bag substantially enclosed within a storage bag. Powdered cell culture media and other constituents are introduced into the mixing bag and are mixed therein. Thereafter, the reconstituted media is conveyed from the mixing bag into the storage bag undergoing sterilization during conveyance therebetween. The reconstituted cell culture media may then be stored in a sterilized condition in the storage bag or maybe dispensed into a cell growth container. In another embodiment of the present invention a plurality of storage bags or mixing bags may be utilized so that reconstituted cell culture media may be both mixed and dispensed while protein-laden cell culture media may be simultaneously harvested back into another of the plurality of storage bags.
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Citations
19 Claims
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1. A containment system for use with cell culture media, the containment system comprising:
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a) a mixing bag having a mixing chamber formed therein; b) a storage bag having a storage chamber formed therein, the storage bag substantially enclosing the mixing bag; c) means for introducing cell culture media constituents into the mixing chamber; d) means for conveying liquid cell culture media from the mixing chamber to the storage chamber; and e) means for dispensing liquid cell culture media from the storage chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A containment system for use in shipping, and dispensing cell culture media, the containment system comprising:
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a) a mixing bag having a mixing chamber formed therein; b) a storage bag having a storage chamber formed therein, the mixing bag residing substantially within the storage bag; and c) means for conveying cell culture media from the mixing bag to the storage bag; and d) means for dispensing cell culture media from the mixing chamber.
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15. A method for reconstituting powdered cell culture media within a containment system, the method comprising the steps of:
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a) introducing the powdered and liquid constituents of cell culture media into a mixing chamber of a mixing bag substantially enclosed by a storage bag; b) mixing to reconstitute the constituents in the mixing chamber; and c) conveying the reconstituted cell culture media from the mixing chamber to the storage chamber. - View Dependent Claims (16, 17)
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18. A containment system for use with cell culture media, the containment system comprising:
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a) a mixing bag having a mixing chamber formed therein; b) an intermediate storage bag having an intermediate storage chamber formed therein, the intermediate storage bag substantially enclosing the mixing bag; c) a storage bag having a storage chamber formed therein, the storage bag substantially enclosing the intermediate storage bag; d) means for conveying liquid cell culture media from the mixing chamber to the intermediate storage chamber; e) means for dispensing liquid cell culture media from the intermediate storage chambers; and f) means for harvesting cell culture media into the storage bag from a cell growth container.
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19. A containment system for use with cell culture media, the containment system comprising:
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a) a mixing bag having a top and a bottom and having a mixing chamber formed therein; b) a storage bag having a top and a bottom and having a storage chamber formed therein, the storage chamber substantially enclosing the mixing bag; c) a dispensing plate affixed through the top of the storage bag, the dispensing plate having an aperture formed therethrough capable of communicating between the storage chamber and the environment outside of the storage bag; d) an introducing plate affixed through the tops of both the mixing bag and the storage bag capable of communicating between the mixing chamber and the environment outside of the storage bag; and e) a pump and tubing communicating between the mixing chamber and the storage chamber.
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Specification