Capillary column packed with glucomannan gel for capillary electrophoresis and methods for making and using same

• US 5,364,520 A
• Filed: 12/10/1992
• Issued: 11/15/1994
• Est. Priority Date: 12/10/1991
• Status: Expired due to Fees

• Current Assignee: Kurita Water Industries Limited
• Original Assignee: Kurita Water Industries Limited
• Inventors: Izumi, Tomonori, Okuyama, Tsuneo, Yamaguchi, Masato
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): DELACROIX MUIRHEI, CYBILLE
• Application Number:
  US07/987,758
• Time in Patent Office: 705 Days
• Field of Search: 204/299 R, 204/182.8, 252/315.1, 252/315.3, 536/114
• US Class Current: 204/605
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture

Abstract

A packing material for capillary electrophoresis comprises capillary electrophoresis in an apparatus having a capillary column, by charging a sample into an end of the capillary column and discharging the sample from the other end of the capillary column, is conducted using a capillary column prepared by packing the column with a packing material comprising glucomannan and then forming a gel in situ from the packed glucomannan. The packing material is packed into the capillary easily and forms a homogeneous gel therein which shows no damage by formation of bubbles and the like and thus can be utilized with stability, has superior health safety and is excellent with respect to biodegradation.

Claims

1. In a gel-packed capillary column adapted for capillary electrophoresis, the improvement wherein the column is packed with a glucomannan gel.

2. A packed capillary column for capillary electrophoresis as claimed in claim 1 wherein the glucomannan is obtained from konjak.

3. A packed capillary column for capillary electrophoresis as claimed in claim 1 wherein the glucomannan has a molecular weight in the range from 50,000 to 2,000,000.

4. In a method of conducting a capillary electrophoresis in a capillary column connected to a source of high voltage and which comprises the steps of charging a sample into an end of the capillary column and discharging the sample from the other end of the capillary column, the improvement wherein the capillary column is a gel-packed column according to claim 1.

5. A method according to claim 4, wherein the gel-packed column is produced by adding an ungelled but gellable buffer solution or dispersion of the glucomannan to the capillary and permitting the solution or dispersion to gel therein.

6. A packed capillary column according to claim 1, wherein the glucomannan has a molecular weight in the range from 1,000,000 to 2,000,000.

7. A packed capillary column according to claim 1, wherein the glucomannan present therein is obtained from a natural source thereof by chemical hydrolysis or enzymatic degradation.

8. A packed capillary column according to claim 1, wherein the glucomannan has a molecular weight in the range of 100,000, 500,000 or 1,000,000 and the concentration of the glucomannan in the gel is in the range of 0.5 to 5 wt/vol %, 0.3 to 2 wt/vol % or 0.1 to 1.5 wt/vol %, respectively.

9. A method of producing a gel-packed capillary column according to claim 1, which comprises the steps of packing the column with a solution or dispersion of glucomannan in a buffer at a concentration suitable for gel electrophoresis and converting the solution or dispersion into a gel in situ in the capillary column.

10. A method of producing the gel-packed capillary column of claim 1, which comprises dissolving the glucomannan in a buffer solution, heating the resultant solution, packing the heated solution in the capillary column and maintaining the packed capillary column at room temperature such that the solution gels.

11. In a gel-packed capillary column adapted for capillary electrophoresis, the improvement wherein the column is packed with a gel of glucomannan obtained from a natural source thereof by chemical hydrolysis or enzymatic degradation and has a molecular weight of 100,000, 500,000 or 1,000,000 and the concentration of the glucomannan in the gel is in the range of 0.5 to 5 wt/vol %, 0.3 to 2 wt/vol % or 0.1 to 1.5 wt/vol %, respectively.