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DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats

  • US 5,364,759 A
  • Filed: 01/31/1991
  • Issued: 11/15/1994
  • Est. Priority Date: 01/31/1991
  • Status: Expired due to Term
First Claim
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1. A DNA profiling assay for detecting polymorphisms in at least one short tandem repeat, comprising the steps of:

  • extracting DNA from a sample to be tested;

    amplifying said at least one short tandem repeat in the extracted DNA, wherein the short tandem repeat sequence is characterized by the formula (Aw Gx Ty Cz)n wherein A,G,T, and C represent the nucleotides;

    w, x, y and z represent the number of each nucleotide and range from 0 to 7;

    the sum of w+x+y+z ranges from 4 to 7; and

    n represents the repeat number and ranges from about 5 to 50; and

    detecting said polymorphisms by identifying said amplified extension products for each different sequence, wherein each different sequence is differentially labelled.

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