Graft copolymers and graft copolymer/protein compositions

• US 5,364,907 A
• Filed: 06/02/1993
• Issued: 11/15/1994
• Est. Priority Date: 10/10/1990
• Status: Expired due to Term

• Current Assignee: Minnesota Mining and Manufacturing Co (3M Company)
• Original Assignee: Minnesota Mining and Manufacturing Co (3M Company)
• Inventors: Coleman, Patrick L., Kotnour, Thomas A., Kangas, Steven L., Rolando, Richard J.
• Primary Examiner(s): Nutter, Nathan M.
• Application Number:
  US08/071,327
• Time in Patent Office: 531 Days
• Field of Search: 525/54.1, 525/69, 525/87, 525/193, 525/204, 525/240, 525/241, 525/242, 525/243, 525/244, 525/260, 525/263, 525/265, 525/327.9, 525/333.3, 525/333.6, 525/375 , 525/435, 525/525, 525/530, 435/188, 530/402, 530/403, 530/811, 530/812, 530/815, 530/816
• US Class Current: 525/54.1
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/264 derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
• CPC Class Codes: B01J 20/28014 characterised by their form
• CPC Class Codes: B01J 20/3007 Moulding, shaping or extruding
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/327 Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3276 Copolymers
• CPC Class Codes: B01J 20/3278 Polymers being grafted on the carrier
• CPC Class Codes: C08F 255/02 on to polymers of olefins having two or three carbon atoms
• CPC Class Codes: C08F 257/02 on to polymers of styrene or alkyl-substituted styrenes
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10S 530/812 Peptides or proteins is immobilized on, or in, an organic carrier
• CPC Class Codes: Y10S 530/815 Carrier is a synthetic polymer
• CPC Class Codes: Y10S 530/816 Attached to the carrier via a bridging agent

Abstract

Graft copolymers comprising a poly-alpha-olefin base polymer selected from the group consisting of polyethylene, polypropylene, polystyrene, and compatible mixtures thereof, having grafted thereto an olefinic monomer. The grafted monomer is present in an amount effective to increase the amount of protein that will bind to the graft copolymer as compared with the base polymer.

Also disclosed are polymer/protein compositions comprising a graft copolymer having a protein immobilized on the surface thereof, processes for the preparation of the above-described graft copolymers and compositions, methods of immobilizing proteins, and methods of immunoassay based on such immobilization.

Claims

1. A graft copolymer consisting essentially of a uniform, homogeneous thermoplastic of

i) polyethylene and

ii) an amount of a grafted monomer selected from the group consisting of 1-vinylimidazole, hydroxyethyl methacrylate, polyethylene glycol monomethacrylate, N-vinyl pyrrolidone, N,N-dimethylacrylamide and mixtures thereof effective to increase protein binding to the graft copolymer as compared with polyethylene.

2. The graft copolymer of claim 1 wherein the graft copolymer comprises about 0.01-20.0 wt. % grafted monomer.

3. The graft copolymer of claim 1 wherein the graft copolymer comprises about 0.5-10.0 wt. % grafted monomer.

4. The graft copolymer of claim 1 in the form of a microtiter well, a test tube, a bead, or a film.

5. A graft copolymer consisting essentially of a uniform, homogeneous thermoplastic of:

i) a base polymer selected from the group consisting of polypropylene, polystyrene, and mixtures thereof and

ii) an amount of 1-vinylimidazole effective to increase protein binding to the graft copolymer as compared with protein binding to only the base polymer, wherein the base polymer is selected from the group consisting of polypropylene, polystyrene, and mixtures thereof.

6. The graft copolymer of claim 5 wherein the graft copolymer comprises about 0.01-20.0 wt. % grafted monomer.

7. The graft copolymer of claim 5 wherein the graft copolymer comprises about 0.5-10.0 wt. % grafted monomer.

8. The graft copolymer of claim 5 in the form of a microtiter well, a test tube, a bead, or a film.