Enhanced maintenance of pregnancy using leukaemia inhibitory factor in embryo culturing

• US 5,366,888 A
• Filed: 04/16/1992
• Issued: 11/22/1994
• Est. Priority Date: 07/09/1990
• Status: Expired due to Fees

• Current Assignee: Zenyth Therapeutics Limited (CSL Limited)
• Original Assignee: Zenyth Therapeutics Limited (CSL Limited)
• Inventors: Parr, Ronald A., Fry, Richard C.
• Primary Examiner(s): Weimar, Elizabeth C.
• Assistant Examiner(s): Stanton, Brian R.
• Application Number:
  US07/838,425
• Time in Patent Office: 950 Days
• Field of Search: 435/69.1, 435/240.1, 435/240.2, 435/240.3, 435/30.1, 435/806, 435/240.21, 600/33, 600/34, 800/2, 800/DIG. 4, 800/6
• US Class Current: 435/375
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 67/027 New or modified breeds of vertebrates
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 5/0604 Whole embryos; Culture medium therefor
• CPC Class Codes: Y10S 435/806 Fertility tests

Abstract

The present invention relates to the use of leukemia inhibitory factor (LIF) in the enhancement of development and maintenance of mammalian embryos, particularly sheep embryos. It has been observed that following the introduction into foster mothers of embryos cultured in vitro in the presence of LIF, the maintenance of pregnancy is enhanced relative to that seen following introduction of embryos that had not been cultured with LIF prior to introduction into foster mothers.

Claims

1. A method of enhancing the maintenance of pregnancy in a mammal into which an embryo has been introduced, wherein said mammal is selected from the group consisting of ruminants, humans, pigs, donkeys, horses, dogs and cats, said method comprising prior to said introducing,

culturing at least one embryo in a medium containing an amount of leukemia inhibitory factor for sufficient time and under appropriate conditions so as to effect an enhancement of the maintenance of pregnancy in said mammal.

2. The method according to claim 1 wherein said mammal is selected from the group consisting of human, sheep, pig, cow, goat, donkey, horse, dog and cat.

3. The method of claim 2 wherein said mammal is a sheep.

4. The method according to claim 1 wherein said LIF is of human or murine origin.

5. The method according to claim 1 wherein said LIF is of sheep, pig, cow, goat, donkey, horse, dog or cat origin.

6. The method according to claim 1, wherein the effective amount of LIF is from about 100 units/ml to about 10,000 units/ml.

7. The method according to claim 6 wherein the effective amount of LIF is from about 500 units/ml to about 5,000 units/ml.

8. The method according to claim 7 wherein the effective amount of LIF is from about 1000 units/ml to about 5,000 units/ml.

9. The method according to claim 1 wherein the medium for maintenance of the embryo is SOF or M2 medium.

10. The method of claim 1 wherein said mammal is a ruminant.

11. The method of claim 10 wherein said ruminant is a sheep.