Treatment of fibrous lignocellulosic biomass by high shear forces in a turbulent couette flow to make the biomass more susceptible to hydrolysis

• US 5,370,999 A
• Filed: 12/17/1992
• Issued: 12/06/1994
• Est. Priority Date: 12/17/1992
• Status: Expired due to Term

• Current Assignee: RA ENERGY, LTD., AFEX CORPORATION, EARNEST STUART, LINDA CLEBOSKI, TOM CAHALAN, BETTY ZOCH
• Original Assignee: Colorado State University Research Foundation (Colorado State University System)
• Inventors: Stuart, Earnest D.
• Primary Examiner(s): Naff, David M.
• Assistant Examiner(s): Meller, Michael V.
• Application Number:
  US07/991,745
• Time in Patent Office: 719 Days
• Field of Search: 435/277, 435/99, 435/105
• US Class Current: 435/99
• CPC Class Codes: C08H 8/00 Macromolecular compounds derived from lignocellulosic materials pretreatment thereof B27N
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: Y02P 20/582 Recycling of unreacted starting or intermediate materials

Abstract

High-shear, microcavitation is used to shred and disintegrate fibrous lignocellulose-containing biomass to make the biomass more susceptible to hydrolysis. This process is preferably done with the biomass particles dispersed in a slurry created using only water. The process can be performed on slurries created with other liquids such as liquid ammonia, aqueous solutions of acid or alkali reagents, or enzyme solutions containing cellulase. High-shear microcavitation is carried out by subjecting the slurry to high-shear forces by injecting the slurry cross currently into a turbulent Couette flow created in a high-frequency, rotor-stator device. This microcavitation shatters the fibrous structure of the biomass in the slurry thereby creating a sheared slurry. The device has a chamber containing a fixed stator equipped with coaxial toothed rings meshing with opposing coaxial toothed rings coupled to a rotor that is coupled to a rotatable shaft.

Claims

1. A method of treating a fibrous lignocellulosic biomass to make it more susceptible to hydrolysis, comprising the following steps:

(a) reducing the particle size of the lignocellulosic biomass to produce a dry flour;

(b) mixing the flour with water to produce a slurry ranging up to about 60% solids; and

(c) subjecting the slurry to high-shear forces by injecting the slurry cross currently into a turbulent Couette flow created in a high-frequency, rotor-stator device under conditions to microcavitate the slurry to shatter the fibrous structure of the biomass in the slurry creating a sheared slurry, said device comprising a chamber containing a fixed stator equipped with coaxial toothed rings meshing with opposing coaxial toothed rings coupled to a rotor that is coupled to a rotatable shaft.

2. The method of claim 1, further comprising:

(d) mixing the sheared slurry from step (c) with an enzyme to form a mixture; and

subjecting the mixture to said high-shear forces in said turbulent Couette flow created by said device under conditions to microcavitate the mixture to form a sheared mixture.

3. The method of claim 2, wherein the enzyme comprises cellulase, cellobiase, or a combination of cellulase and cellobiase.

4. The method of claim 2, wherein the mixture further comprises a nonionic surfactant.

5. The method of claim 4, wherein the surfactant possesses a polyoxyethylene glycol group.

6. The method of claim 2, further comprising:

(e) separating the sheared mixture from step (d) producing a filtrate and a retentate;

adding the retentate to a newly formed sheared slurry from step (c) forming a mixture; and

simultaneously adding an enzyme to the mixture and subjecting the mixture to said high-shear forces in said turbulent Couette flow created by said device under conditions to microcavitate the mixture.

7. A method of treating lignocellulosic biomass to make it more susceptible to hydrolysis, comprising the steps of:

(a) mixing a lignocellulosic biomass flour with water to produce a slurry; and

(b) subjecting the slurry to high-shear forces by injecting the slurry cross currently into a turbulent Couette flow created in a high-frequency, rotor-stator device under conditions to microcavitate the slurry to shatter the fibrous structure of the biomass in the slurry creating a sheared slurry, said device comprising a chamber containing a fixed stator equipped with coaxial toothed rings meshing with opposing coaxial toothed rings coupled to a rotor that is coupled to a rotatable shaft.

8. The method of claim 7, further comprising:

(c) simultaneously adding an enzyme and a surfactant to the sheared slurry from step (b), and subjecting the sheared slurry to said high-shear forces in said turbulent Couette flow created by said device under conditions to microcavitate the sheared slurry.

9. The method of claim 8, wherein the enzyme comprises cellulase, cellobiase, or a combination of cellulase and cellobiase.

10. The method of claim 8, wherein the surfactant comprises a nonionic surfactant having a polyoxyethylene glycol group.

11. The method of claim 8, further comprising:

(d) separating the sheared slurry from step (c) producing a filtrate and a retentate;

(e) adding the retentate to a newly formed sheared slurry from step (b) to form a mixture; and

simultaneously adding an enzyme and a surfactant to the mixture and subjecting the mixture to said high-shear forces in said turbulent Couette flow created by said device under conditions to microcavitate the mixture.

12. A method of treating lignocellulosic biomass to make it more susceptible to hydrolysis, comprising the following steps:

(a) mixing a lignocellulosic biomass flour with water, an enzyme capable of converting cellulose and hemicellulose to sugars, and a surfactant to form a slurry; and

(b) subjecting the slurry to high-shear forces by injecting the slurry cross currently into a turbulent Couette flow created in a high-frequency, rotor-stator device under conditions to microcavitate the slurry to shatter the fibrous structure of the biomass in the slurry creating a sheared slurry, said device comprising a chamber containing a fixed stator equipped with coaxial toothed rings meshing with opposing coaxial toothed rings coupled to a rotor that is coupled to a rotatable shaft.

13. The method of claim 12, wherein steps (a) and (b) are performed simultaneously.

14. The method of claim 12, wherein the enzyme comprises cellulase, cellobiase, or a combination of cellulase and cellobiase.

15. The method of claim 12, wherein the surfactant comprises a nonionic surfactant having a polyoxyethylene glycol group.

16. The method of claim 12, further comprising:

(c) separating the sheared slurry from step (b) producing a filtrate and a retentate;

(d) adding the retentate to a newly formed sheared slurry from step (b) forming a mixture; and

simultaneously adding an enzyme and a surfactant to the mixture and subjecting the mixture to said high-shear forces in said Couette flow created by said device.

17. A method of treating lignocellulosic biomass to make it more susceptible to hydrolysis, comprising the following steps:

(a) mixing a lignocellulosic biomass flour with water, an enzyme capable of converting cellulose and hemicellulose to sugars, and a surfactant to form a slurry;

(b) subjecting the slurry using high-shear forces by injecting the slurry cross currently into a turbulent Couette flow created in a high-frequency, rotor-stator device under conditions to microcavitate the slurry to shatter the fibrous structure of the biomass in the slurry creating a sheared slurry, said device comprising a chamber containing a fixed stator equipped with coaxial toothed rings meshing with opposing coaxial toothed rings coupled to a rotor that is coupled to a rotatable shaft; and

(c) mixing the sheared slurry from step (b) with the slurry from step (a) and repeating step (b).