Apparatus for transporting substances into living cells and tissues

• US 5,371,015 A
• Filed: 01/09/1991
• Issued: 12/06/1994
• Est. Priority Date: 11/13/1984
• Status: Expired due to Term

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Sanford, John C., Wolf, Edward D., Allen, Nelson K.
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US07/637,954
• Time in Patent Office: 1,427 Days
• Field of Search: 435/287, 435/172.1, 435/172.3, 935/52, 935/53, 935/85, 604/68-70, 128/740, 73/11, 73/12, 73/167, 124/60, 102/501, 102/502, 102/508-510
• US Class Current: 435/285.3
• CPC Class Codes: C12M 35/00 Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12N 15/8207 by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

Inert or biologically active particles are propelled at cell at a speed whereby the particles penetrate the surface of the cells and become incorporated into the interior of the cells. The process can be used to mark cells or tissue or to biochemically affect tissues or tissue in situ as well as single cells in vitro. Apparatus for propelling the particles toward target cells or tissues are also disclosed. A method for releasing particles adhered to a rotor device is also disclosed.

Claims

1. An apparatus for introducing one or more particles into at least one cell which comprises said one or more particles associated with means to accelerate said one or more particles to thereby cause said one or more particles to penetrate the at least one cell and whereby at least a portion of said one or more particles becomes incorporated into the interior of the at least one cell, wherein said one or more particles comprise biological substances and have a diameter sufficiently small to penetrate the at least one cell without killing the at least one cell.

2. The apparatus as set forth in claim 1 further comprising means associated with said means to accelerate in order to direct the accelerated one or more particles toward the at least one cell.

3. An apparatus as in claim 1, wherein said means to accelerate comprises discharge means having a periphery and a solid substrate carrying said one or more particles, wherein said discharge means imparts momentum to said solid substrate carrying said one or more particles which in turn accelerates said one or more particles.

4. An apparatus as in claim 3, wherein said solid substrate comprises a macroprojectile carrying said one or more particles, and said apparatus further comprises a macroprojectile stopping means, located contiguous the periphery, to stop said macroprojectile while allowing said one or more particles to continue toward the at least one cell.

5. An apparatus as in claim 3, further comprising a substrate stopping means located contiguous the periphery, to stop said substrate carrying said one or more particles while allowing said one or more particles to continue toward the at least one cell.

6. An apparatus as in claim 1, wherein said means to accelerate comprises mechanical impulse means.