Method of photometric in vitro determination of the content of an analyte in a sample

• US 5,371,020 A
• Filed: 05/07/1993
• Issued: 12/06/1994
• Est. Priority Date: 09/19/1991
• Status: Expired due to Term

• Current Assignee: Radiometer A/S (Danaher Corporation)
• Original Assignee: Radiometer A/S (Danaher Corporation)
• Inventors: Frischauf, Peter A.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/050,100
• Time in Patent Office: 578 Days
• Field of Search: 436/68, 436/164, 436/165, 436/171, 422/58, 422/82.05, 422/82.06, 422/82.09, 422/82.11, 356/39, 356/40, 356/246, 356/434, 356/320, 356/440
• US Class Current: 436/165
• CPC Class Codes: G01N 2021/036 transformable, modifiable
• CPC Class Codes: G01N 2021/0364 flexible, compressible
• CPC Class Codes: G01N 2021/3129 Determining multicomponents by multiwavelength light
• CPC Class Codes: G01N 2035/1062 for testing the liquid while it is in the transfer device
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 2201/0662 Comparing measurements on two or more paths in one sample

Abstract

A method of photometric in vitro determination of the content of an analyte in a sample is disclosed. The sample is located in a measuring chamber which has a radiation path length and has at least one at least partially transparent wall part. The measuring chamber is in optical communication with an optical system adapted for the analyte which includes a radiation source and a radiation detector. The measuring chamber is adjustable in shape which enables the radiation path length across the measuring chamber to be changed. In a first measuring step an unknown first radiation path length across the measuring chamber is set and radiation at at least two wavelengths is transmitted from the radiation source through the measuring chamber and to the radiation detector. In a second step, the measuring chamber is adjusted in shape thereby setting a second unknown path length across the measuring chamber. Radiation at the same wavelengths as during the first step is again transmitted from the radiation source through the measuring chamber and to the radiation detector. The analyte content is then determined on the basis of radiation detected in each of the measuring steps.

Claims

1. A method of photometric in vitro determination of the content of an analyte in a sample, the method comprising:

A) providing a measuring chamber for holding the sample, the measuring chamber comprising at least one partially transparent wall in optical communication with the sample, wherein the measuring chamber receives radiation through the at least one partially transparent wall and transmits the radiation over a path having a path length through the sample and out of the chamber through the at least one partially transparent wall, a shape of the measuring chamber being adjustable for varying the path length through the chamber;

B) providing an optical system comprising a radiation source and a radiation detector;

C) mounting the measuring chamber in optical communication with the radiation source for transmitting radiation into the sample through the at least one partially transparent wall, and mounting the measuring chamber in optical communication with the radiation detector for receiving the radiation transmitted through the sample;

D) setting in a first measuring step a first unknown radiation path length through the measuring chamber, and transmitting radiation at at least two wavelengths from the radiation source through the measuring chamber and to the radiation detector;

E) in a second measuring step, adjusting the shape of the measuring chamber and thereby setting a second unknown radiation path length across the measuring chamber, and transmitting radiation at the same at least two wavelengths as used during the first measuring step from the radiation source through the measuring chamber and to the radiation detector; and

F) determining the analyte content of the sample from the absorbance of the sample detected in each of the measuring steps, the determination being independent of the change in path length, the determination being made by solving simultaneous equations.

2. The method according to claim 1 wherein the measuring chamber has optical transmission properties, the optical transmission properties being essentially independent of the shape adjustment of the measuring chamber.

3. The method according to claim 1 wherein the measuring chamber comprises two at least partially transparent opposite walls along the radiation path, the radiation being received through one wall, and transmitted out of the other wall.

4. The method according to claim 3 wherein the adjustment in shape of the measuring chamber comprises displacement of one of the two opposite at least partially transparent walls.

5. The method according to claim 3 wherein the adjustment in shape of the measuring chamber comprises displacement of each of the two opposite at least partially transparent walls.

6. The method according to claim 1 wherein the measuring chamber comprises a wall opposite the at least one partially transparent wall, the opposite wall reflecting incoming radiation back through the at least one partially transparent wall.

7. The method according to claim 6 wherein the adjustment in shape of the measuring chamber comprises displacement of the at least one partially transparent wall.

8. The method according to claim 6 wherein the adjustment in shape of the measuring chamber comprises displacement of the opposite wall.

9. The method according to claim 6 wherein the adjustment in shape of the measuring chamber comprises displacement of each of the opposite wall and the at least one partially transparent wall.

10. The method according to claim 1 wherein the transmitted radiation is wide-band radiation, and wherein one of the at least two wavelengths is a wavelength at which the analyte absorbs.

11. The method according to claim 1 wherein the analyte is CO₂.

12. The method according to claim 11 further comprising transmitting and detecting radiation at three wavelengths during each of the measuring steps, wherein the wavelengths are approximately 4228 nm, 4268 nm and 4308 nm, each having a band width of approx. 20 nm.

13. The method according to claim 1 further comprising transmitting and detecting radiation at more than two wavelengths during each of the measuring steps.

14. An analyzer for performing photometric in vitro determination of the content of an analyte in a sample, the analyzer comprising:

A) a measuring chamber for holding the sample, the measuring chamber comprising at least one partially transparent wall in optical communication with the sample, wherein the measuring chamber receives radiation through the at least one partially transparent wall and transmits the radiation over a path having a path length through the sample and out of the chamber through the at least one partially transparent wall, a shape of the measuring chamber being adjustable for varying the path length through the chamber;

B) an optical system comprising

i) a radiation source for generating at least two wavelengths of radiation, the at least one partially transparent wall of the measuring chamber being mounted in optical communication with the radiation source for receiving radiation at the at least two wavelengths from the source into the sample; and

ii) a radiation detector for detecting the at least two wavelengths of radiation, the at least one partially transparent wall of the measuring chamber being mounted in optical communication with the radiation detector for transmitting the radiation of the at least two wavelengths through the sample and to the detector;

C) means for setting in a first measuring step a first unknown radiation path length through the measuring chamber, the radiation source transmitting radiation at the at least two wavelengths through the measuring chamber, the radiation detector detecting the radiation at the at least two wavelengths;

D) means for adjusting the shape of the measuring chamber and thereby setting a second unknown radiation path length across the measuring chamber in a second measuring step, the radiation source transmitting radiation at the same at least two wavelengths as during the first measuring step across the measuring chamber, the radiation detector detecting the radiation at the same at least two wavelengths; and

E) means for determining the analyte content of the sample from the absorbance of the sample detected in each of the measuring steps by solving simultaneous equations, the determination being independent of the change in path length.

15. The analyzer according to claim 14, wherein the means for adjusting the shape of the measuring chamber comprises:

the radiation source or radiation detector being movable and being movable, the interacting with the movable wall to adjust the shape of the chamber.

16. The analyzer according to claim 14, wherein the measuring chamber has optical transmission properties, the optical transmission properties being essentially independent of the shape of the measuring chamber.

17. The analyzer according to claim 14, wherein the measuring chamber comprises two at least partially transparent opposite walls along the radiation path, the radiation being received through one wall, and transmitted out of the other wall.

18. The analyzer according to claim 17, wherein one of the two opposite at least partially transparent walls is movable, and the means for adjusting the shape of the measuring chamber comprises means for displacing the movable of the two at least partially transparent walls.

19. The analyzer according to claim 17, wherein each of the two opposite at least partially transparent walls is movable, and the means for adjusting the shape of the measuring chamber comprises means for displacing each of the movable opposite at least partially transparent walls.

20. The analyzer according to claim 14, wherein the measuring chamber comprises a wall opposite the at least one partially transparent wall, the opposite wall reflecting incoming radiation back through the at least one partially transparent wall.

21. The analyzer according to claim 20, wherein the at least one partially transparent wall is movable, and the means for adjusting the shape of the measuring chamber comprises means for displacing the at least one partially transparent wall.

22. The analyzer according to claim 20, wherein the opposite wall is movable, and the means for adjusting the shape of the measuring chamber comprises means for displacing the opposite wall.

23. The analyzer according to claim 20, wherein each of the opposite wall and the at least one partially transparent wall is movable, and the means for adjusting the shape of the measuring chamber comprises means for displacing each of the opposite wall and the at least one partially transparent wall.

24. The analyzer according to claim 14, wherein the transmitted radiation is wide-band radiation, and wherein one of the at least two wavelengths is a wavelength at which the analyte absorbs.

25. The analyzer according to claim 14, wherein the analyte is CO₂.

26. The analyzer according to claim 25, wherein the radiation source is adapted for transmitting radiation at three wavelengths through the measuring chamber during each of the measuring steps, and the radiation detector is adapted for detecting the radiation at the same three wavelengths during each of the measuring steps, and wherein the three wavelengths are approximately 4228 nm, 4268 nm and 4308 nm, each having a band width of approx. 20 nm.

27. The analyzer according to claim 14, wherein the radiation source is adapted for transmitting radiation at more than two wavelengths through the measuring chamber during each of the measuring steps, and the radiation detector is adapted for detecting the radiation at each of the more than two wavelengths during each of the measuring steps.