Surface plasmon resonance device and method of determining biological, biochemical, or chemical analyte

• US 5,374,563 A
• Filed: 04/09/1993
• Issued: 12/20/1994
• Est. Priority Date: 09/01/1990
• Status: Expired due to Fees

• Current Assignee: Fisons PLC (Sanofi )
• Original Assignee: Fisons PLC (Sanofi )
• Inventors: Maule, Coin H.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US07/984,430
• Time in Patent Office: 620 Days
• Field of Search: 356/128, 356/317, 356/318, 356/369, 356/435, 356/68.1, 422/82.05, 422/88, 422/164, 436/165, 436/169, 436/170, 436/805, 436/807, 435/4, 435/7.8, 435/7.1, 435/808
• US Class Current: 436/165
• CPC Class Codes: G01N 21/21 Polarisation-affecting properties G01N21/19 takes precedence
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

A sensor based on the technique of surface plasmon resonance (SPR) comprises an SPR device, a source of electromagnetic radiation from which radiation can be directed onto the device, and a detector to measure the intensity of radiation reflected from the SPR device. The electromagnetic radiation directed onto the SPR device contains both Transverse Electric-polarized and Transverse Electric-polarized components. A polarization analyzer is interposed between the device and the detector such that, at angles away from resonance, little or no light reaches the detector. The sensor is particularly useful in the qualitative and/or quantitative determination of biological, biochemical or chemical analytes.

Claims

1. A surface plasmon resonance (SPR) sensor, comprising

(a) an SPR device in the form of a block of dielectric material having a first surface, and a metallic material applied to said first surface of said block so as to create a metallic-dielectric boundary,

(b) a source of electromagnetic radiation from which electromagnetic radiation can be directed onto said boundary such that total internal reflection occurs,

(c) a detector to measure the intensity of electromagnetic radiation reflected from said SPR device, and

(d) a polarization analyzer interposed between said SPR device and said detector such that, at angles resonance, little or no electromagnetic radiation reaches said detector,

wherein said source of electromagnetic radiation is arranged such that the electromagnetic radiation directed onto said boundary contains TE(Transverse Electric)-polarized and TM(Transverse Magnetic)-polarized components.

3. A sensor as claim in claim 1, wherein said polarization analyzer comprises a polarizer arranged such that its transmission axis is orthogonal to the resultant polarization of radiation reflected from said boundary.

4. A sensor as claimed in claim 1, wherein the radiation directed onto said boundary contains approximately equal amounts of the TE- and TM-polarized components.

5. A sensor as claimed in claim 1, further comprising a phase compensator located between said source of electromagnetic radiation and said polarization analyzer.

6. A sensor as claimed in claim 1, wherein said block is of glass.

7. A sensor as claimed in claim 1, wherein said layer of metallic material is of silver.

9. A sensor as claimed in claim 1, wherein said source of electromagnetic radiation is a laser.

10. A sensor as claimed in claim 1, wherein said detector is a position-sensitive detector.

2. A sensor for the qualitative or quantitative determination of a biological, biochemical or chemical analyte, which sensor comprises

(a) a surface plasmon resonance device in the form of a block of material having a first surface, a layer of metallic material applied to at least part of said first surface forming a metallic-dielectric boundary, and a layer of material sensitive to the analyte applied to said layer of metallic material,

(b) a source of electromagnetic radiation, said electromagnetic radiation being directed onto said block in such a way as to be reflected off said boundary, and said source of electromagnetic radiation being arranged such that the electromagnetic radiation contains TE(Transverse Electric)-polarized and TM(Transverse polarized components,

(c) a detector for measuring the intensity of radiation reflected from said boundary, and

(d) a polarization analyzer interposed between said block and said detector such that, at angles away resonance, little or no electromagnetic detector.

8. A sensor as claimed in claim 2, wherein said layer of material sensitive to the analyte is a layer of biomolecules immobilized upon said layer of metallic material.

11. A method for the quantitative or qualitative determination of a biological, biochemical or chemical analyte in a sample, which method comprises the steps of

(a) contacting the sample with the sensitive area of a surface plasmon resonance (SPR) device in the form of a block of material, which block has a layer of metallic material applied to at least part of a first surface thereof forming a metallic-dielectric boundary, the sensitive area being a layer of material sensitive to the analyte coated on the metallic layer,

(b) directing electromagnetic radiation into the block in such a way that said radiation is reflected off said boundary, said radiation containing both TE(Transverse Electric)- polarized and TM(Transverse Magnetic)-polarized components, and

(c) measuring with a detector the intensity of radiation reflected from the SPR device and passing through a polarization analyzer interposed between the SPR device and the detector.