Method for determining the biodistribution of substances using fluorescence spectroscopy
First Claim
1. A method for determining the biodistribution of a substance in a body comprising:
- a) administering an effective amount of a substance capable of light-induced fluorescence to tissue to be examined and to tissue whose condition is known;
b) exciting said tissue to be examined and said tissue whose condition is known with a beam of light from a light source to cause said substance to emit fluorescence;
c) measuring said fluorescence emitted from said tissue to be examined and said tissue whose condition is known each at a first wavelength and a second wavelength;
d) calculating a first fluorescence intensity ratio for said examined tissue and a second intensity ratio for said tissue whose condition is known, wherein said intensity ratios are said fluorescence at said first wavelength divided by said fluorescence at said second wavelength; and
e) comparing said first intensity ratio with said second intensity ratio and thereby determining the biodistribution of said substance.
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Abstract
A method is disclosed for determining the biodistribution of substances using fluorescence spectroscopy. A photosensitizing agent or other intrinsically fluorescent agent, or an agent labeled with an extrinsic fluorophor is administered to a subject. A fiberoptic probe integrated with an excitation light source illuminates the examined tissue and causes fluorescence. The fluorescence is recorded by a spectrograph and plotted as a spectral curve. The intensity ratio (S1/S2) for the fluorescence from the photosensitizing agent (S1) and autofluorescence (S2) for the examined tissue is used as an index for drug presence and compared with the intensity ratio at the same wavelengths for various tissues.
940 Citations
10 Claims
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1. A method for determining the biodistribution of a substance in a body comprising:
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a) administering an effective amount of a substance capable of light-induced fluorescence to tissue to be examined and to tissue whose condition is known; b) exciting said tissue to be examined and said tissue whose condition is known with a beam of light from a light source to cause said substance to emit fluorescence; c) measuring said fluorescence emitted from said tissue to be examined and said tissue whose condition is known each at a first wavelength and a second wavelength; d) calculating a first fluorescence intensity ratio for said examined tissue and a second intensity ratio for said tissue whose condition is known, wherein said intensity ratios are said fluorescence at said first wavelength divided by said fluorescence at said second wavelength; and e) comparing said first intensity ratio with said second intensity ratio and thereby determining the biodistribution of said substance. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification
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- Resources
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Current AssigneeCedars-Sinai Medical Center (Cedars Sinai Health System)
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Original AssigneeCedars-Sinai Medical Center (Cedars Sinai Health System)
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InventorsGrundfest, Warren S., Vari, Sandor G.
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Primary Examiner(s)Green, Randall L.
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Assistant Examiner(s)ZUTTARELLI, ANTHONY
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Application NumberUS08/039,991Time in Patent Office644 DaysField of Search606/2, 606/13-15, 128/633-634, 128/664-665US Class Current600/317CPC Class CodesA61B 10/0233 Pointed or sharp biopsy ins...A61B 18/22 the beam being directed alo...A61B 2017/00061 spectrumA61B 2017/22087 photodynamicA61B 2090/062 penetration depthA61B 2090/306 using optical fibresA61B 2090/3941 Photoluminescent markersA61B 5/0059 using light, e.g. diagnosis...A61B 5/415 the glands, e.g. tonsils, a...A61B 5/416 the spleenA61B 5/418 lymph vessels, ducts or nodesG01N 21/6428 Measuring fluorescence of f...
