Optical fiber pH microsensor and method of manufacture

• US 5,378,432 A
• Filed: 10/07/1993
• Issued: 01/03/1995
• Est. Priority Date: 10/16/1990
• Status: Expired due to Term

• Current Assignee: Puritan Bennett Corp. (Medtronic Plc)
• Original Assignee: Puritan Bennett Corp. (Medtronic Plc)
• Inventors: Hahn, Soonkap, Bankert, Charles S., Hui, Henry K.
• Primary Examiner(s): Snay, Jeffrey R.
• Application Number:
  US08/132,794
• Time in Patent Office: 453 Days
• Field of Search: 422/68.1, 422/82.06, 422/82.07, 422/82.08, 422/82.09, 436/68, 436/163, 436/165, 436/172, 128/634, 128/636, 356/39, 8/647, 385/12, 427/2, 427/163-165
• US Class Current: 422/82.07
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/773 Porous polymer jacket; Polymer matrix with indicator
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 31/221 for investigating pH value

Abstract

The optical fiber pH microsensor includes an optical fiber having a portion of the surface of a light conducting core covered with a layer of a pH sensitive dye material. The dye material is covalently bonded to a polymeric matrix which is in turn covalently bonded to the optical fiber core to prevent leaching of the indicator dye material during extended use. The dye material is crosslinked in situ over the tip of the optical fiber to yield a hydrophilic, ion permeable pH sensor which can be used intravascularly to monitor blood

Claims

1. An analyte sensor, comprising:

a light conducting optical fiber having an outer surface;

an analyte sensing matrix crosslinked in situ on the surface of the light conducting optical fiber, the analyte sensing matrix including a crosslinked polyether polyamine polymer and a fluorescent dye indicator material covalently bonded to said crosslinked polyether polyamine polymer, with said crosslinked polyether polyamine polymer covalently bonded to said outer surface of said light conducting optical fiber.

2. The sensor of claim 1, wherein said crosslinked polyether polyamine covalently bonded to said dye indicator material is crosslinked with a polyether polyisocyanate.

3. The sensor of claim 1, further including a coating of reflective material applied over the analyte sensing matrix.

4. The sensor of claim 1, wherein said fluorescent dye indicator material comprises hydroxypyrenetrisulfonic acid.

5. A microsensor for measuring pH in a fluid, comprising:

a sensor means including a light transmitting optical fiber having a bonding surface;

a pH sensing matrix crosslinked in situ on the bonding surface of the light transmitting optical fiber for receiving an excitation light signal transmitted by said light transmitting optical fiber, the pH sensing matrix including a flourescent dye indicator material for emitting an output light signal carried by said light transmitting optical fiber, said fluorescent dye indicator material being covalently bonded to a polyether polymaine and a polyether polyisocyanate crosslinked to said polyether polyamine, said polyether being polyamine covalenty bonded to said bonding surface of said light transmitting optical fiber; and

a coating of reflective material applied over the pH sensing matrix.

6. The microsensor of claim 5, wherein said coating of reflective material comprises titanium dioxide.

7. The microsensor of claim 5, wherein said fluorescent dye indicator material comprises hydroxypyrenetrisulfonic acid.

8. The microsensor of claim 5, wherein said coating of reflective material comprises a mixture of approximately 50% titanium dioxide with the remainder comprising polyether polyisocyanate.

9. A method of making an analyte sensor, comprising the steps of:

covalently bonding a fluorescent dye indicator material to a polyether polyamine; and

crosslinking said polyether polyamine in situ on a portion of a surface of a light conducting optical fiber to covaneltly bond said analyte sensing polymeric matrix to said portion of the surface of said light conducting optical fiber to form an analyte sensing polyermic matrix.

10. The method of claim 9, wherein said polyether polyamine is crosslinked with a polyether polyisocyanate.

11. A method of claim 9, further including the step of applying a coating of reflective material over the analyte sensing polymeric matrix.