Wash composition for determination of microorganisms associated with periodontal diseases

• US 5,378,629 A
• Filed: 06/07/1993
• Issued: 01/03/1995
• Est. Priority Date: 10/08/1991
• Status: Expired due to Fees

• Current Assignee: Eastman Kodak Company
• Original Assignee: Eastman Kodak Company
• Inventors: Boyer, Bradley P., Snyder, Brian A., Contestable, Paul B.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Le, Long V.
• Application Number:
  US08/072,029
• Time in Patent Office: 575 Days
• Field of Search: 436/17, 436/18, 436/518, 436/826, 436/534, 436/8, 435/962, 435/967, 435/970
• US Class Current: 436/17
• CPC Class Codes: G01N 33/531 Production of immunochemical test materials
• CPC Class Codes: G01N 33/56955 involved in periodontal diseases
• CPC Class Codes: Y10S 435/962 Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
• CPC Class Codes: Y10S 435/967 Standards, controls, materials, e.g. validation studies, buffer systems
• CPC Class Codes: Y10S 435/975 Kit
• CPC Class Codes: Y10S 436/826 Additives, e.g. buffers, diluents, preservatives
• CPC Class Codes: Y10T 436/10 Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]

Abstract

An aqueous wash composition has been found useful in methods for determination of specific binding ligands. The composition is buffered to a pH of less than or equal to 6 or greater than or equal to 9. It also includes as its essential component at least about 0.1 weight percent of an anionic surfactant which is represented by the formula:

[A-SO.sub.3+y ].sup.-(1+y) [X.sup.+m ].sub.n

wherein A is a hydrocarbon having a molecular weight of at least about 180, X^+m is hydrogen or a monovalent or divalent cation, m is 1 or 2, is 0 or 1, and n is 1 or 2 provided that m and n are not both 2. Optionally and preferably, the wash composition also includes a nonimmunoreactive protein. This wash composition is particularly useful in methods for determination of microorganisms associated with periodontal diseases. Such methods can be of a variety of formats, but immunometric assays are particularly useful. The wash composition can be included as part of a diagnostic test kit.

Claims

1. An aqueous wash composition for specific binding assays buffered to a pH of less than or equal to about 6 or greater than or equal to about 9, said composition comprising:

at least about 0.1 weight percent of an anionic surfactant which is represented by the formula;

space="preserve" listing-type="equation">(A--SO.sub.3+y).sup.-(l+y) (X.sup.+m).sub.n

wherein A is an alkyl or alkyl-substituted phenyl having a molecular weight of from about 180 to about 250, X^+m is hydrogen or a monovalent or divalent cation, m is 1 or 2, y is 0 or 1, and n is 1 or 2 provided that m and n are not both 2.

2. The composition of claim 1 buffered to a pH of from about 9.5 to about 11.

3. The composition of claim 1 comprising from about 1 to about 5 weight percent of said surfactant.

4. The composition of claim 1 further comprising a nonimmunoreactive protein.

5. The composition of claim 4 wherein said nonimmunoreactive protein is selected from the group consisting of:

casein or another milk proteins, serum proteins and fibrinogen.

6. The composition of claim 1 wherein A is alkyl having from 14 to 16 carbon atoms, m and y are both 1, and n is 2.

7. The composition of claim 6 wherein A is tetradecyl, and X^+m is a sodium or potassium ion.

8. An aqueous wash composition for immunoassays buffered to a pH of from about 9.5 to about 11, said composition comprising:

from about 1 to about 5 weight percent of an anionic surfactant which is represented by the formula;

space="preserve" listing-type="equation">(A--SO.sub.3+y).sup.-(l+Y) (X.sup.+m).sub.n

wherein A is tetradecyl, X^+m is a sodium or potassium ion, m and y are both 1, and n is 2.