Multifunctional filtration apparatus

• US 5,380,437 A
• Filed: 02/02/1993
• Issued: 01/10/1995
• Est. Priority Date: 02/02/1993
• Status: Expired due to Fees

• Current Assignee: Biomedical Research and Development Laboratories Incorporated
• Original Assignee: Biomedical Research and Development Laboratories Incorporated
• Inventors: Bertoncini, Joseph
• Primary Examiner(s): KIM, SUN U
• Application Number:
  US08/012,515
• Time in Patent Office: 707 Days
• Field of Search: 210/232, 210/252, 210/323.1, 210/247, 210/258, 210/341, 210/406, 210/416.1, 210/455, 210/456, 141/8, 141/65, 73/863.23, 73/863.32, 73/863.33, 73/864.11, 73/864.35, 422/101, 422/102, 422/103, 422/104, 435/296, 435/312, 435/311
• US Class Current: 210/416.1
• CPC Class Codes: B01D 29/05 supported
• CPC Class Codes: B01D 29/52 in parallel connection
• CPC Class Codes: B01D 29/78 for washing
• CPC Class Codes: B01D 29/96 in which the filtering elements are moved between filtering operations; Particular measures for removing or replacing the filtering elements; Transport systems for filters B01D29/09, B01D29/70 take precedence
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids

Abstract

A multifunctional filtration apparatus is provided for the automated transfer, separation and filtration which accommodates all types of filter papers or filter mediums including aerophobic filter mediums such as nitrocellulose, nylon, biological membranes as well as aerophyllic filter mediums. The automated multifunctional filtration apparatus includes a first manifold and one or more separation chambers disposed on one side of a filter medium and a second manifold disposed on the other side of the filter medium. The multifunctional filtration apparatus provides for the high speed transfer, separation of gaseous and liquid components of a fluid before filtration without cross contamination of multiple samples by utilizing the first manifold to transfer fluids containing filtrable substances from test tubes or sample plates to a separation chamber disposed above the filter medium where the gases and liquids are separated and the gases removed before the utilization of the second manifold to provide for the filtration of the filterable substances on the filter medium. Subsequent addition of wash fluids or reagents for further reaction of the filterable substances can also be accomplished utilizing the novel multifunctional filtration apparatus. As a result the automated multifunctional filtration apparatus can be used for all types of filter paper or filter mediums used such as paper, glass, fiber synthetic and traditional filter papers as well as biological membranes or filter mediums having reactive or reactable surfaces for filtering and reacting captured filterable substances.

Claims

1. A multifunctional filtration apparatus comprising:

(a) a first manifold;

(b) a second manifold;

(c) a separation chamber disposed between said first manifold and said second manifold;

(d) a filter support disposed between said separation chamber and said second manifold; and

(e) a valve having a vacuum inlet and a first outlet and a second outlet, said first outlet operatively connected to said first manifold or said separation chamber and said second outlet operatively connected to said second manifold.

2. The multifunctional filtration apparatus of claim 1 further comprising an injector having one end disposed outside said first manifold and the other end disposed in said separation chamber for injecting fluids in said separation chamber.

3. The multifunctional filtration apparatus of claim 2 wherein said injector extends from about 25 to 90 percent of the length of said separation chamber.

4. The multifunctional filtration apparatus of claim 2 further comprising a fluid pick up needle operatively connected to said injector.

5. The multifunctional filtration apparatus of claim 4 further comprising a wash or reagent addition needle operatively connected to a wash manifold for adding wash or reagent fluids wherein said wash or reagent addition needle is in a distanced relationship to said fluid pick up needle.

6. The multifunctional filtration apparatus of claim 5 further comprising a purge conduit operatively connected to said wash manifold and said valve.

7. The multifunctional filtration apparatus of claim 6 further comprising a pump for pumping wash or reagent fluids to said wash manifold.

8. The multifunctional filtration apparatus of claim 1 wherein said separation chamber is divided into a plurality of separation chambers.

9. The multifunctional filtration apparatus of claim 8 wherein said plurality of separation chambers is at least four.

10. The multifunctional filtration apparatus of claim 8 wherein said plurality of separation chambers is at least twenty-four.

11. The multifunctional filtration apparatus of claim 8 wherein said plurality of separation chambers is at least forty-eight.

12. The multifunctional filtration apparatus of claim 8 wherein said plurality of separation chambers is at least ninety-six.

13. The multifunctional filtration apparatus of claim 8 wherein said plurality of separation chambers is at least three hundred eighty-four.

14. The multifunctional filtration apparatus of claim 1 wherein said valve includes means for selectively connecting said vacuum inlet with said first outlet and said second outlet.

15. The multifunctional filtration apparatus of claim 14 further comprising an injector for injecting fluids having one end disposed outside said first manifold and the other end disposed in said separation chamber.

16. The multifunctional filtration apparatus of claim 15 wherein said injector extends from about 25 to 90 percent of the length of said separation chamber.

17. The multifunctional filtration apparatus of claim 14 further comprising a fluid pick up needle operatively connected to said injector.

18. The multifunctional filtration apparatus of claim 17 further comprising a wash manifold operatively connected to a fluid addition needle wherein said fluid addition needle is in a distanced relationship to said fluid pick up needle.

19. The multifunctional filtration apparatus of claim 18 further comprising a purge conduit operatively connected to said wash manifold and said valve.

20. The multifunctional filtration apparatus of claim 19 further comprising a pump for pumping fluids to said wash manifold.

21. The multifunctional filtration apparatus of claim 20 wherein said separation chamber is divided into a plurality of separation chambers and said injector is divided into a plurality of injectors and said fluid pick up needle is divided into a plurality of fluid pick up needles and said fluid addition needle is divided into a plurality of fluid addition needles.

22. The multifunctional filtration apparatus of claim 21 wherein said plurality of separation chambers is at least four and said plurality of injectors is at least four and said plurality of fluid pick up needles is at least four and said plurality of fluid addition needles is at least four.

23. The multifunctional filtration apparatus of claim 21 wherein said plurality of separation chambers is at least twenty-four and said plurality of injectors is at least twenty-four and said plurality of fluid pick up needles is at least twenty-four and said plurality of fluid addition needles is at least twenty-four.

24. The multifunctional filtration apparatus of claim 21 wherein said plurality of separation chambers is at least forty-eight and said plurality of injectors is at least forty-eight and said plurality of pick up needles is at least forty-eight and said plurality of fluid addition needles is at least forty-eight.

25. The multifunctional filtration apparatus of claim 21 wherein said plurality of separation chambers is at least ninety-six and said plurality of injectors is at least ninety-six and said plurality of pick up needles is at least ninety-six and said plurality of fluid addition needles is at least ninety-six.

26. A filtration apparatus for transferring and filtering fluids comprising:

(a) a first layer having a first manifold and a separation chamber;

(b) a second layer having a filter support and a second manifold;

(c) means for detachably joining said first layer to said second layer; and

(d) a valve having a vacuum inlet and a first outlet and a second outlet, said first outlet operatively connected to said first layer and said second outlet operatively connected to said second layer.

27. The filtration apparatus for transferring and filtering fluids of claim 26 further comprising an injector in said first layer for injecting fluid in said separation chamber.

28. The filtration apparatus for transferring and filtering fluids of claim 27 further comprising a plurality of fluid pick up needles operatively connected to a plurality of injectors.

29. The filtration apparatus for transferring and filtering fluids of claim 28 further comprising a wash manifold operatively connected to a plurality of wash addition needles wherein said wash addition needles are in a distanced relationship to said fluid pick up needles.

30. The filtration apparatus for transferring and filtering fluids of claim 29 further comprising a purge conduit operatively connected to said wash manifold and said valve.

31. The filtration apparatus for transferring and filtering fluids of claim 30 further comprising a wash manifold valve and a solenoid for opening and closing said wash manifold valve.

32. The filtration apparatus for transferring and filtering fluids of claim 30 further comprising a support structure for holding said fluid pick up needles and said wash addition needles in a distanced relationship to one another.

33. The filtration apparatus for transferring and filtering fluids of claim 30 wherein said filter support and said second manifold are separable from each other in said second layer.

34. The filtration apparatus for transferring and filtering fluids of claim 33 further comprising a liquid injector disposed in said filter support for injecting liquids into said second manifold.

35. The filtration apparatus for transferring and filtering fluids of claim 34 wherein said second manifold includes means for collecting liquids injected in said second manifold from said liquid injector.

36. The filtration apparatus for transferring and filtering fluids of claim 30 further comprising means for providing said vacuum inlet of said valve simultaneous access to said first outlet and said second outlet.

37. The filtration apparatus for transferring and filtering fluids of claim 36 wherein said simultaneous access to said first outlet can provide a vacuum pressure P₁ and said simultaneous access to said second outlet can provide a vacuum pressure P₂ wherein said vacuum pressure P₂ is greater than said vacuum pressure P₁.

38. The filtration apparatus for transferring and filtering fluids of claim 36 wherein said valve includes a second vacuum inlet and means for connecting said vacuum inlet with said first outlet and said second vacuum inlet and said second outlet.

39. The filtration apparatus for transferring and filtering fluids of claim 30 wherein said second layer includes a sample tray recess.

40. The filtration apparatus for transferring and filtering fluids of claim 39 further comprising a filter support insert for filling said sample tray recess.

41. The filtration apparatus for transferring and filtering fluids of claim 30 wherein said separation chamber in said first layer is divided into a plurality of separation chambers.

42. The filtration apparatus for transferring and filtering fluids of claim 41 further comprising a plurality of wash addition needles, a plurality of fluid pick up needles and a plurality of injectors.

43. The filtration apparatus for transferring and filtering fluids of claim 42 wherein said plurality of separation chambers are at least twenty-four and said plurality of wash addition needles are at least twenty-four and said plurality of fluid pick up needles are at least twenty-four and said plurality of injectors is at least twenty-four.

44. The filtration apparatus for transferring and filtering fluids of claim 42 wherein said plurality of separation chambers are at least forty-eight and said plurality of wash addition needles are at least forty-eight and said plurality of fluid pick up needles are at least forty-eight and said plurality of injectors is at least forty-eight.

45. The filtration apparatus for transferring and filtering fluids of claim 44 wherein said second layer includes a forty-eight well sample tray recess.

46. The filtration apparatus for transferring and filtering fluids of claim 42 wherein said plurality of separation chambers are at least ninety-six and said plurality of wash addition needles are at least ninety-six and said plurality of fluid pick up needles are at least ninety-six and said plurality of injectors is at least ninety-six.

47. The filtration apparatus for transferring and filtering fluids of claim 46 wherein said second layer includes a ninety-six well sample tray recess.

48. A filtration apparatus for filtering through all types of filter mediums comprising:

(a) a first layer having a first manifold;

(b) a second layer having a separation chamber;

(c) a third layer providing a filter support medium;

(d) a fourth layer having a second manifold;

(e) means for detachably opening and closing said second layer with respect to said third layer; and

(f) a valve having a vacuum inlet and a first outlet and a second outlet, said first outlet operatively connected to said first layer and said second outlet operatively connected to said fourth layer.

49. The filtration apparatus for filtering through all types of filter mediums of claim 48 further comprising an injector having one end disposed outside said first layer and the other end disposed in said separation chamber.

50. The filtration apparatus for filtering through all types of filter mediums of claim 49 further comprising a fluid pick up needle operatively connected to said injector.

51. The filtration apparatus for filtering through all types of filter mediums of claim 50 further comprising a wash manifold operatively connected to a wash addition needle wherein said wash addition needle is in a distanced relationship to said fluid pick up needle.

52. The filtration apparatus for filtering through all types of filter mediums of claim 51 further comprising a purge conduit operatively connected to said wash manifold and said valve.

53. The filtration apparatus for filtering through all types of filter mediums of claim 52 further comprising a housing for holding a plurality of fluid pick up needles and a plurality of wash addition needles in a distanced relationship to one another.

54. The filtration apparatus for filtering through all types of filter mediums of claim 53 wherein said third layer and said fourth layer include means for detachably opening and closing said third layer and said fourth layer.

55. The filtration apparatus for filtering through all types of filter mediums of claim 53 wherein said third layer includes a sample tray recess.

56. The filtration apparatus for filtering through all types of filter mediums of claim 55 further comprising a filter support insert for filling said sample tray recess.

57. The filtration apparatus for filtering through all types of filter mediums of claim 53 wherein said separation chamber in said second layer is divided into a plurality of separation chambers.

58. The filtration apparatus for filtering through all types of filter mediums of claim 57 further comprising a plurality of wash addition needles, a plurality of fluid pick up needles and a plurality of injectors.

59. The filtration apparatus for filtering through all types of filter mediums of claim 58 wherein said plurality of separation chambers are at least twenty-four and said plurality of wash addition needles are at least twenty-four and said plurality of fluid pick up needles are at least twenty-four and said plurality of injectors is at least twenty-four.

60. The filtration apparatus for filtering through all types of filter mediums of claim 58 wherein said plurality of separation chambers are at least forty-eight and said plurality of wash addition needles are at least forty-eight and said plurality of fluid pick up needles are at least forty-eight and said plurality of injectors is at least forty-eight.

61. The filtration apparatus for filtering through all types of filter mediums of claim 60 wherein said second layer includes a forty-eight well sample tray recess.

62. The filtration apparatus for filtering through all types of filter mediums of claim 58 wherein said plurality of separation chambers are at least ninety-six and said plurality of wash addition needles are at least ninety-six and said plurality of fluid pick up needles are at least ninety-six and said plurality of injectors is at least ninety-six.

63. The filtration apparatus for filtering through all types of filter mediums of claim 62 wherein said second layer includes a ninety-six well sample tray recess.