Apparatus for pumping and directing fluids for hematology testing

• US 5,380,491 A
• Filed: 01/21/1993
• Issued: 01/10/1995
• Est. Priority Date: 01/21/1993
• Status: Expired due to Term

• Current Assignee: Drew Scientific Holdings, Inc.
• Original Assignee: CDC Technologies, Inc. (Escalon Medical Corporation)
• Inventors: DeCava, David C., Carver, Edward L. Jr.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Carpenter, Robert
• Application Number:
  US08/007,111
• Time in Patent Office: 719 Days
• Field of Search: 422/73, 422/81, 422/231, 604/224, 417/568, 435/291
• US Class Current: 422/73
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

An apparatus for hematology testing, which has a sensing unit defining a counting orifice for the flow of a blood sample through the counting orifice to analyze the blood sample, and a pump unit having three syringes. A first syringe is coupled in fluid communication with the sensing unit on the inlet side of the counting orifice for injecting a stream of blood sample through the counting orifice. A second syringe is coupled in fluid communication with the sensing chamber on the inlet side of the counting orifice for simultaneously injecting a sheath of fluid surrounding the sample stream on the inlet side of the counting orifice. And a third syringe is coupled to the sensing chamber on the outlet side of the counting orifice for aspirating a sheath of fluid from the sensing chamber surrounding the sample stream on the outlet side of the counting orifice.

Claims

1. An apparatus for hematology testing, comprising:

a sensing unit defining a counting orifice for the flow of a blood sample through the sensing unit to analyze the blood sample, the counting orifice defining an inlet side and an outlet side, and the sensing unit including a first inlet port and a second inlet port, the first and second inlet ports each being fluidly coupled to and located on the inlet side of the counting orifice; and

means for pumping including at least two syringes, a first syringe being coupled by a sample line to the first inlet port of the sensing unit and injecting a blood sample from the inlet side through the counting orifice, and a second syringe being located upstream of the second inlet port of the sensing unit and coupled by a sheath line to the second inlet port and simultaneously injecting a sheath of fluid through the sheath line and second inlet port and surrounding the sample stream on the inlet side of the counting orifice.

2. An apparatus as defined in claim 1, wherein the pump means includes a drive motor coupled to both the first and second syringes for simultaneously actuating the syringes.

3. An apparatus as defined in claim 2, wherein the drive motor is coupled to a threaded shaft and the threaded shaft is coupled to the first and second syringes, whereupon rotation of the drive motor causes rotation of the threaded shaft and simultaneous actuation of the first and second syringes.

4. An apparatus as defined in claim 3, wherein the drive motor is a stepping motor.

5. An apparatus as defined in claim 3, wherein the pump means further includes a drive plate coupled between the first and second syringes and the threaded shaft, whereupon rotation of the shaft by the drive motor causes the drive plate to move in the axial direction of the shaft and simultaneously actuate the first and second syringes.

6. An apparatus as defined in claim 1, further including a first-position sensor located approximately at one extreme of permissible movement of the first and second syringes and a second-position sensor located at another approximate extreme of permissible movement of the first and second syringes, each sensor generating a signal when at least one of the first and second syringes reaches the respective extreme position of permissible movement.

7. An apparatus as defined in claim 1, further comprising a valve matrix coupled between the pump means and the sensing unit for controlling the flow of fluids between the pump means and the sensing unit.

8. An apparatus as defined in claim 1, further comprising a control unit coupled to the pump means and the sensing unit for controlling actuation of the syringes.

9. An apparatus as defined in claim 8, further comprising a first lysing agent chamber and a second lysing agent chamber, each being coupled to the pump means for aspirating the lysing agents into at least one syringe of the pump means.

10. An apparatus as defined in claim 9, wherein the control unit includes a database pertaining to predetermined quantities of lysing agents necessary for formulating blood/reagent mixtures for a plurality of species, and is responsive to an input indicating a specific species to control the pump means to aspirate into at least one of the first and second syringes predetermined quantities of the lysing agents from the first and second lysing chambers corresponding to the respective species.

11. An apparatus as defined in claim 9, further comprising a mixing chamber coupled in fluid communication with the pump means for receiving the lysing agents injected by at least one syringe of the pump means for mixing the lysing agents with a blood sample.

12. An apparatus as defined in claim 1, further comprising a sample probe coupled to at least one syringe of the pump means for aspirating a predetermined volume of blood sample into the probe for testing.

13. An apparatus as defined in claim 1, wherein the sensing unit defines an inlet chamber coupled in fluid communication with the counting orifice, the first inlet port and the second inlet port, and at least a portion of the second inlet port is oriented substantially on a tangent to a surface defining the inlet chamber for injecting the sheath fluid in a generally spiral path surrounding the sample stream.

14. An apparatus as defined in claim 1, wherein the sensing unit includes a third inlet port coupled in fluid communication with the outlet side of the counting orifice, and a sheath tube coupled in fluid communication with the third inlet port for at least one of injecting and aspirating sheath fluid through the sheath tube surrounding the sample stream on the outlet side of the counting orifice.

15. An apparatus as defined in claim 14, wherein the sensing unit further defines an exit chamber coupled in fluid communication with the outlet side of the counting orifice for receiving the mixture of sheath fluid and sample fluid injected through the counting orifice, and the third inlet port is coupled in fluid communication with the exit chamber, and at least a portion of the third inlet port is oriented substantially on a tangent to a surface defining the exit chamber for directing the sheath fluid in a generally spiral path surrounding the sample stream on the outlet side of the counting orifice.

16. An apparatus as defined in claim 1, wherein the sensing unit further comprises an outlet port fluidly coupled to the outlet side of the counting orifice, and the pump means further comprises a third syringe fluidly coupled to the outlet port for aspirating the fluids injected through the counting orifice from the outlet side of the counting orifice.

17. An apparatus for hematology analysis, comprising:

a sensing unit defining an orifice for receiving a sample stream of blood cells and a sheath of diluent surrounding the sample stream injected into the sensing unit, and including a first inlet port and a second inlet port, each being located on and fluidly coupled to the inlet side of the counting orifice, and a third port fluidly coupled with the outlet side of the counting orifice; and

three positive-displacement pumps, a first pump coupled by a sample line to the first inlet port on the inlet side of the orifice and injecting the sample stream into the sensing unit on the inlet side of the orifice, a second pump located upstream from the second inlet port and coupled by a sheath line to the second inlet port on the inlet side of the orifice and injecting a first sheath of diluent through the sheath line and second inlet port and surrounding the sample stream on the inlet side of the orifice, and a third pump coupled with the third port on the outlet side of the orifice which is capable of injecting or aspirating a second sheath of diluent at the outlet side of the orifice surrounding the sample stream exiting the orifice.

18. An apparatus as defined in claim 17, further including means for directing at least one of the first and second sheaths of diluent in a substantially spiral path surrounding the sample stream.

19. An apparatus as defined in claim 18, wherein the means for directing includes the second inlet port oriented substantially on a tangent to a surface defining a chamber on the inlet side of the orifice.

20. An apparatus as defined in claim 18, wherein the means for directing includes the third port oriented substantially on a tangent to a surface defining a chamber on the outlet side of the orifice.

21. An apparatus as defined in claim 17, further comprising a control unit coupled to at least one of the three positive-displacement pumps, and including a database pertaining to predetermined quantities of reagents corresponding to a plurality of blood/reagent mixtures for a plurality of species, the control unit being responsive to an input selecting a particular species resulting in at least one pump aspirating the predetermined volumes of reagents as desired for each of said respective species.

22. An apparatus as defined in claim 21, further comprising a first chamber containing a first reagent and a second chamber containing a second reagent, the first and second chambers each are fluidly coupled with at least one pump for aspirating reagent from each chamber into at least one pump, and a mixing container fluidly coupled with at least one pump for injecting the predetermined quantities of reagents from at least one pump into the mixing container.

23. An apparatus for particle analysis, comprising:

a sensing unit defining a counting orifice, the counting orifice defining an inlet side and an outlet side for receiving sample fluid through the counting orifice and analyzing particles within the sample fluid;

first means for injecting a stream of sample fluid through the inlet side of the counting orifice; and

second means for injecting a sheath of fluid into the sensing unit on the inlet side of the counting orifice and surrounding the sample fluid injected through the inlet side of the counting orifice, the second means being located upstream from the inlet side of the counting orifice.

24. An apparatus as defined in claim 23, wherein the sensing unit includes a first inlet port coupled in fluid communication with the inlet side of the counting orifice, and a second inlet port coupled in fluid communication with the inlet side of the counting orifice, and the first means for pumping includes a first positive-displacement pump coupled in fluid communication with the first inlet port for injecting a stream of sample fluid through the inlet side of the counting orifice, and the second means for pumping includes a second positive-displacement pump coupled in fluid communication with the second inlet port for injecting a sheath of fluid surrounding the sample fluid through the inlet side of the counting orifice.

25. An apparatus as defined in claim 23, further comprising third means for pumping coupled in fluid communication with the outlet side of the counting orifice and for aspirating fluids injected through the counting orifice through the outlet side of the counting orifice.

26. An apparatus as defined in claim 25, wherein the sensing unit includes an outlet port coupled in fluid communication with the outlet side of the counting orifice, and the third means includes a positive-displacement pump coupled in fluid communication with the outlet port for aspirating fluids injected through the counting orifice through the outlet side of the counting orifice.

27. An apparatus as defined in claim 23, further comprising fourth means for pumping coupled in fluid communication with the outlet side of the counting orifice and for injecting or aspirating sheath fluid on the outlet side of the counting orifice and surrounding the sample stream exiting the counting orifice.

28. An apparatus for particle analysis of a sample stream surrounded by a sheath of fluid, comprising a sensing unit defining a counting orifice;

and an inlet chamber coupled in fluid communication with the counting orifice;

an outlet chamber coupled in fluid communication with the opposite side of the counting orifice relative to the inlet chamber;

means for injecting a substantially uniform sample stream through a sheath of fluid, including a first inlet port coupled in fluid communication with the counting orifice and oriented substantially coaxial with the counting orifice for injecting a sample stream of fluid through the center of the counting orifice; and

means for preventing intermixing of the sample stream and sheath of fluid including a second inlet port coupled in fluid communication with the inlet chamber and oriented substantially tangential to a contiguous surface defining the inlet chamber for injecting a stream of sheath fluid into the inlet chamber substantially tangential to the surface defining the inlet chamber and forming the sheath of fluid surrounding the sample stream injected through the counting orifice.

29. An apparatus as defined in claim 28, further comprising another inlet port coupled in fluid communication with the inlet chamber and oriented substantially tangential to a contiguous surface defining the inlet chamber for injecting a stream of fluid into the inlet chamber substantially tangential to the surface defining the inlet chamber.

30. An apparatus as defined in claim 28, further comprising another inlet port coupled in fluid communication with the outlet chamber and oriented substantially tangential to a contiguous surface defining the outlet chamber for injecting or aspirating a stream of fluid into the outlet chamber flowing along the surface defining the outlet chamber and surrounding the sample stream exiting the counting orifice.

31. An apparatus as defined in claim 30, further comprising a pump unit coupled in fluid communication with the additional inlet port for injecting or aspirating fluid through the respective inlet port into the outlet side of the counting orifice surrounding the sample stream exiting the counting orifice.

32. An apparatus as defined in claim 28, further comprising an outlet port coupled in fluid communication with the outlet chamber and oriented substantially coaxial with the counting orifice for receiving fluid flowing through the outlet chamber.

33. An apparatus as defined in claim 28, further comprising a first positive-displacement pump coupled in fluid communication with the first inlet port for injecting a sample stream of fluid through the first inlet port and the counting orifice.

34. An apparatus as defined in claim 28, further comprising a second positive-displacement pump coupled in fluid communication with at least one of the second and third inlet ports for injecting a stream of fluid through the respective inlet port substantially tangential to the contiguous surface defining the inlet chamber.