Biosensor and method of quantitative analysis using the same

• US 5,382,346 A
• Filed: 10/20/1993
• Issued: 01/17/1995
• Est. Priority Date: 05/17/1991
• Status: Expired due to Term

• Current Assignee: Kyoto Daiichi Kagaku Company Limited
• Original Assignee: Kyoto Daiichi Kagaku Company Limited
• Inventors: Uenoyama, Harumi, Okuda, Hisashi
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US08/138,263
• Time in Patent Office: 454 Days
• Field of Search: 204/403, 204/418, 204/415, 435/817
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance or its related substance, in which the biologically active material or an optionally used mediator is placed at a part which is remote from a position of an electrode which acts as electrochemical detector means, and optionally the biological active material or the mediator is covered with a polymer layer.

Claims

1. A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance, wherein the biologically active material is placed at a part which is remote from a position of an electrode which-acts as electrochemical detector means.

2. The biosensor according to claim 1, which comprises a mediator which is placed at a part which is remote from said position of said electrode together with said biologically active substance.

3. The biosensor according to claim 1, which comprises a mediator which is provided on said electrode.

4. The biosensor according to claim 1, wherein said biologically active substance is bound with a hydrophilic polymer.

5. The biosensor according to claim 3, wherein said mediator is covered with a layer of a hydrophilic polymer.

6. The biosensor according to claim 1, wherein said biologically active substance is an enzyme.

7. The biosensor according to claim 6, wherein said enzyme is an oxidoreductase.