Use of sparingly soluble salt of a heteropoly acid for the determination of an analyte, a corresponding method of determination as well as a suitable agent thereof

• US 5,382,523 A
• Filed: 04/13/1993
• Issued: 01/17/1995
• Est. Priority Date: 12/02/1989
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Hoenes, Joachim, Unkrig, Volker, Wielinger, Hans
• Primary Examiner(s): Wityshyn, Michael G.
• Assistant Examiner(s): Leary, Louise N.
• Application Number:
  US08/045,203
• Time in Patent Office: 644 Days
• Field of Search: 435/14, 435/4, 435/18, 435/25, 252/435, 436/113, 436/111, 436/112
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10T 436/173845 Amine and quaternary ammonium
• CPC Class Codes: Y10T 436/174614 Tertiary amine
• CPC Class Codes: Y10T 436/175383 Ammonia

Abstract

A sparingly soluble salt of a heteropoly acid is used for the determination of an analyte which is an aromatic amine rich in electrons or together with another substance leads to such an amine. The analyte is determined by means of the heteropoly blue color formed.

Claims

1. A reagent for the determination of an analyte comprising:

a substance or substances capable of reacting with the analyte to form an organic amine which is a stronger reducing agent than aniline, and

a sparingly soluble salt of a heteropoly acid which forms heteropoly blue with the organic amines wherein the cation of the sparingly soluble salt of the heteropoly acid is larger than NH₄ +.

2. A reagent according to claim 1, wherein the heteropoly acid is a heteropoly acid of molybdenum, molybdenum and tungsten, vanadium and molybdenum or vanadium, molybdenum and tungsten with phosphorus, arsenic, silicon or germanium as the heteroatoms.

3. A reagent according to claim 2, wherein the heteropoly acid of molybdenum is 18-molybdodiphosphoric acid.

4. A reagent according to claim 1, wherein the cation is the formula R¹ R² R³ R⁴ X⁺ in which R¹, R², R³ and R⁴ are the same or different and denote hydrogen, or an alkyl, aryl or aralkyl residue, with the proviso that all of R¹, R², R³ and R⁴ are not hydrogen, or two alkyl residues may be taken together to form an alkylene residue, and X represents a phosphorus or a nitrogen atom.

5. A reagent according to claim 4, wherein X is a nitrogen atom.

6. A reagent according to claim 1 wherein the amine has the formula II ##STR2## in which R⁵ denotes hydroxy or amino, in which the amino group is optionally substituted once or twice by an alkyl residue, and the alkyl residue is itself optionally substituted by a hydroxy group, an amino group optionally substituted once or twice by alkyl, PO₃ H₂, SO₃ H or CO₂ H, and R⁶ and R⁷ which are both the same or different and represent hydrogen or alkyl, in which the alkyl residue is optionally substituted by a hydroxy group, an amino group optionally substituted once or twice by alkyl, PO₃ H₂, SO₃ H or CO₂ H, and R⁸, R⁹, R¹⁰ and R¹¹ are the same or different and denote hydrogen, alkyl, alkoxy, alkylthio, aryloxy, arylthio, halogen, carboxy, carboxyalkyl or alkoxycarbonyl.

7. A reagent according to claim 1 which is in a solution.

8. A reagent according to claim 1 which is on a carrier.