Apparatus for monitoring hematocrit levels of blood

• US 5,385,539 A
• Filed: 06/30/1992
• Issued: 01/31/1995
• Est. Priority Date: 06/30/1992
• Status: Expired due to Fees

• Current Assignee: LifeStream International, Inc. (Getinge AB)
• Original Assignee: Advanced Haemotechnologies
• Inventors: Maynard, David L.
• Primary Examiner(s): RIMELL, SAMUEL G
• Application Number:
  US07/906,926
• Time in Patent Office: 945 Days
• Field of Search: 356/39, 356/40, 356/41, 250/338.1, 250/345, 128/632, 128/633, 128/634, 604/4, 604/5, 604/6
• US Class Current: 604/6.08
• CPC Class Codes: A61M 2205/331 used as turbidity change detectors, e.g. for priming-blood or plasma-hemoglubine-interface detection
• CPC Class Codes: A61M 2205/3313 used specific wavelengths
• CPC Class Codes: G01J 2001/1647 one signal maintained constant
• CPC Class Codes: G01N 2015/055 for hematocrite determination
• CPC Class Codes: G01N 2021/1738 Optionally different kinds of measurements; Method being valid for different kinds of measurement
• CPC Class Codes: G01N 2021/4709 Backscatter
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence
• CPC Class Codes: G01N 2201/0621 Supply
• CPC Class Codes: G01N 2201/0662 Comparing measurements on two or more paths in one sample

Abstract

An apparatus and method are provided for measuring the hematocrit level of blood. The presently preferred embodiment comprises a light emitting device which emits an amount of light into a blood sample. This light travels through the blood sample to two light detecting devices positioned relative to the light emitting device in a predetermined geometry such that light emitted from the light emitting device must travel farther to reach one of the light detecting devices than to reach the other, thereby forming a light path from the light emitting device to one light detecting device which is longer than the path from the light emitting device to the other light detecting device. According to the present invention, the amount of light detected by one of the light detecting devices is regulated so that the amount of light detected is constant. Thereafter, the amount of light detected by the unregulated light detecting device is a linear representation of the hematocrit of the blood in the blood sample. The hematocrit sensor may be used within a plasma separator apparatus wherein the hematocrit measurement regulates the operating parameters of the autotransfusion system to maintain the hematocrit of the blood within a predetermined range.

Claims

1. An apparatus for measuring the hematocrit of blood comprising

light emitting means for emitting light into a blood sample;

first light detecting means for detecting light emitted from the light emitting means, said first light detecting means positioned to receive light emitted from the light emitting means into the blood sample along a first path from the light emitting means, through the blood sample, to the first light detecting means, the first light detecting means outputting a signal corresponding to the amount of light detected;

second light detecting means for detecting light emitted from the light emitting means, said second light detecting means being positioned to receive light emitted from the light emitting means into the blood sample, such that light emitted from the light emitting means must travel farther to reach the second light detecting means than to reach the first light detecting means, thereby forming a second path from the light emitting means, through the blood sample, to the second light detecting means which is longer than the first path from the light emitting means to the first light detecting means, the second light detecting means outputting a signal corresponding to the amount of light detected; and

light intensity regulating means for regulating the intensity of light emitted by the light emitting means such that the received light on one of the paths remains at a constant value.

2. An apparatus for measuring the hematocrit of blood as defined in claim 1, wherein the light emitting means comprises an infrared light emitting diode.

3. An apparatus for measuring the hematocrit of blood as defined in claim 1, wherein the light emitting means comprises an infrared laser.

4. An apparatus for measuring the hematocrit of blood as defined in claim 2, wherein the infrared light emitting diode emits light at approximately 805 nanometers.

5. An apparatus for measuring the hematocrit of blood as defined in claim 1, wherein at least one of the light detecting means comprises a PIN diode.

6. An apparatus for measuring the hematocrit of blood as defined in claim 1, further comprising amplifying means for providing an amplified signal which is a linear representation of the hematocrit of blood in the blood sample.

7. An apparatus for measuring the hematocrit of blood as defined in claim 1, wherein at least one of the light detecting means comprises a photodetector.

8. An apparatus for measuring the hematocrit of blood as defined in claim 1, further comprising:

first conveying means, in communication with the light emitting means, for transmitting light from the light emitting means to the blood;

second conveying means, in communication with the first light detecting means, for transmitting light passing through the blood to the first light detecting means along the first path; and

third conveying means, in communication with the second light detecting means, for transmitting light passing through the blood to the second light detecting means along the second path.

9. An apparatus for measuring the hematocrit of blood as defined in claim 8, wherein the conveying means comprises glass fibers.

10. An apparatus for measuring the hematocrit of blood as defined in claim 8, wherein the conveying means comprises plastic fibers.

11. An apparatus for measuring the hematocrit of blood as defined in claim 1, further comprising an optical connector, said optical connector comprising a generally hollow member adapted to receive therein the blood sample with respect to which hematocrit is to be measured, and an optically clear flexible optical window positioned on the generally hollow member through which blood can be monitored.

12. An apparatus for measuring the hematocrit of blood as defined in claim 11, further comprising a support extending from the flexible optical window substantially perpendicularly from the hollow member, for securing to the hollow member the light emitting and light detecting means, thereby allowing the light emitting and light detecting means access to the blood sample without direct contact with the blood sample.

13. An apparatus for measuring the hematocrit of blood as defined in claim 11, wherein the flexible optical window comprises a polyvinylchloride resin material.

14. An apparatus for measuring the hematocrit of blood as defined in claim 11, wherein the flexible optical window comprises an optically clear material.

15. An apparatus for measuring the hematocrit of blood comprising:

light emitting means for emitting light into a blood sample;

first light detecting means the detecting light emitted from the light emitting means, said first light detecting means positioned to receive light emitted from the light emitting means into the blood sample along a first path from the light emitting means, through the blood sample. to the first light detecting means, said first light detecting means outputting a signal corresponding to the amount of light detected;

second light detecting means for detecting light emitted from the light emitting means. said second light detecting means being positioned to receive light emitted from the light emitting means into the blood sample, such that light emitted from the light emitting means must travel farther to reach the second light detecting means than to reach the first light detecting means, thereby forming a second path from the light emitting means, through the blood sample, to the second light detecting means which is longer than the first path from the light emitting means to the first light detecting means, the second light detecting means outputting a signal corresponding to the amount of light detected;

first conveying means, in communication with the light emitting means, for transmitting light from the light emitting means to the blood;

second conveying means, in communication with the near detecting means, for transmitting to the first light detecting means light passing through the blood along the first path;

third conveying means, in communication with the far detecting means, for transmitting to the second light detecting means light passing through the blood along the second path; and

light intensity regulating means for regulating the intensity of light emitted by the light emitting means such that the received light on one of the paths remains at a constant value.

16. An apparatus for measuring the hematocrit of blood as defined in claim 15, wherein the light emitting means comprises an infrared light emitting diode.

17. An apparatus for measuring the hematocrit of blood as defined in claim 15, wherein the light detecting means comprises a photodiode.

18. An apparatus for measuring the hematocrit of blood as defined in claim 15, wherein the conveying means comprises glass fibers.

19. An apparatus for measuring the hematocrit of blood as defined in claim 15, wherein the conveying means comprises plastic fibers.

20. An apparatus for measuring the hematocrit of blood as defined in claim 15, further comprising amplifying means for performing offset and gain calibration of the signals output from the first and second light detecting means so as to provide an amplification signal which is a linear representation of the hematocrit of blood in the blood sample.

21. An optical connector for use with a hematocrit sensor comprising:

a generally hollow member adapted to receive a blood sample therein with respect to which hematocrit is to be measured; and

an optically clear flexible optical window positioned on the generally hollow member, and through which the blood sample within the generally hollow member may be visually accessed for measurement of its hematocrit;

wherein the optical connector presses firmly into the flexible optical window to permit a substantially tight fitting contact.

22. An optical connector as defined in claim 21, wherein the flexible optical window comprises a polyvinylchloride resin material.

23. An apparatus for measuring the hematocrit of blood as defined in claim 21, further comprising a support extending from the flexible optical window substantially perpendicularly from the generally hollow member for securing to the hollow member a hematocrit sensor so as to allow measurement of the hematocrit of the blood sample within the generally hollow member.

24. An apparatus for regulating the operation of a plasma separator apparatus to keep the hematocrit measurement of blood output therefrom within a predetermined range, comprising:

a plasma separator apparatus for removing unwanted waste components from the blood in order to cleanse the blood; and

monitoring means for measuring the hematocrit of the cleansed blood outputted from the plasma separator apparatus, and for thereafter automatically regulating operating parameters of the plasma separator apparatus so as to maintain a hematocrit of the blood within a predetermined range;

wherein the monitoring means for automatically regulating operating parameters of the plasma separator apparatus comprises a hematocrit sensor for measuring the hematocrit of blood, interconnected with the plasma separator apparatus by a microcomputer, said hematocrit sensor comprising light emitting means for emitting light into a blood sample, first light detecting means for detecting light emitted from the light emitting means, said first light detecting means positioned to receive light emitted from the light emitting means, through the blood sample, along a first path, and the first light detecting means outputting a signal corresponding to the amount of light detected, second light detecting means detecting light emitted from the light emitting means, said second light detecting means being positioned to receive light emitted from the light emitting means, through the blood sample, along a second path, such that light emitted from the light emitting means must travel farther to reach the second detecting means than to reach the first light detecting means, thereby forming a second path from the light emitting means to the second light detecting means which is longer than the first path from the light emitting means to the first light detecting means, and light intensity regulating means for regulating the intensity of light emitted by the light emitting means such that the received light in one of the paths remains at a constant value.

25. An apparatus for regulating the operation of a plasma separator apparatus to keep the measurement of blood output therefrom within a predetermined range, comprising:

a plasma separator apparatus removing unwanted waste components from the blood in order to cleanse the blood; and

monitoring means for measuring the hematocrit of the cleansed blood outputted from the plasma separator apparatus, and for thereafter automatically regulating operating parameters of the plasma separator apparatus so as to maintain a hematocrit of the blood within a predetermined range;

wherein the monitoring means for automatically regulating operating parameters of the plasma separator apparatus comprises a hematocrit sensor for measuring the hematocrit of blood, interconnected with the plasma separator apparatus by limit switches, said hematocrit sensor comprising light emitting means for emitting light into a blood sample, first light detecting means for detecting light emitted from the light emitting means, said first light detecting means positioned to receive light emitted from the light emitting means, through the blood sample, along a first path, and the first light detecting memos outputting a signal corresponding to the amount of light detected, second light detecting means for detecting light emitted from the light emitting means, said second light detecting means being positioned to receive light emitted from the light emitting mean, through the blood sample, along a second path, such that light from the light emitting means must travel further to reach the second detecting means than to reach the first light detecting means, thereby forming a second path from the light emitting means to the second light detecting means which is longer than the first path from the light emitting means to the first light detecting means, and light intensity regulating means for regulating the intensity of light emitted by the light emitting means such that the received light on one of the paths remains at a constant value.