Biosensor and method for hematocrit determination
First Claim
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1. An apparatus for measuring the hematocrit level of a blood sample, comprising:
- (a) a first insulating substrate;
(b) working and counter electrodes affixed to the first insulating substrate;
(c) a second insulating substrate, which overlays the working and counter electrodes, has a window for exposing a portion of the working and counter electrodes, and has a cut out portion at one end to allow contact between the electrodes and a meter and a power source; and
(d) a porous substrate, which is impregnated with an electroactive compound, overlays the window, and is spatially displaced from the working and counter electrodes so that there is an empty space between the working and counter electrodes and the porous substrate prior to addition of the blood sample to the apparatus.
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Abstract
A biosensor and method for determining the hematocrit level of a whole blood sample using electrochemistry. The biosensor includes working and counter electrodes. A porous membrane is impregnated with an electroactive compound and is spatially displaced from the surface of the electrodes. When a whole blood sample is applied to the porous substrate, a mixture of the electroactive compound and the blood is formed. The mixture settles on the electrodes and a potential difference is applied sufficient to oxidize or reduce the electroactive compound and generate a current. This current can be measured and correlated to hematocrit level.
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18 Claims
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1. An apparatus for measuring the hematocrit level of a blood sample, comprising:
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(a) a first insulating substrate; (b) working and counter electrodes affixed to the first insulating substrate; (c) a second insulating substrate, which overlays the working and counter electrodes, has a window for exposing a portion of the working and counter electrodes, and has a cut out portion at one end to allow contact between the electrodes and a meter and a power source; and (d) a porous substrate, which is impregnated with an electroactive compound, overlays the window, and is spatially displaced from the working and counter electrodes so that there is an empty space between the working and counter electrodes and the porous substrate prior to addition of the blood sample to the apparatus. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification