Method for activation of polyanionic fluorescent dyes in low dielectric media with quaternary onium compounds
First Claim
1. A method for activating the fluorescence of a polyanionic fluorescent dye in low dielectric media comprisinga. incorporating said fluorescent dye in a low dielectric medium, such dye being quenched in such medium,b. adding an activation compound including a quaternary onium compound which interacts with the quenched fluorophore in a manner to restore the fluorescence and create an activated fluorophore in the low dielectric medium, andc. measuring the fluorescence or fluorescent-related parameter generated.
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Abstract
The invention pertains to a method for generating the fluorescence of polyanionic fluorophores in low dielectric media with fluorescence activation produced by specific quaternary onium compounds. The method may be used for measuring an analyte and may be useful in sensor devices.
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18 Claims
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1. A method for activating the fluorescence of a polyanionic fluorescent dye in low dielectric media comprising
a. incorporating said fluorescent dye in a low dielectric medium, such dye being quenched in such medium, b. adding an activation compound including a quaternary onium compound which interacts with the quenched fluorophore in a manner to restore the fluorescence and create an activated fluorophore in the low dielectric medium, and c. measuring the fluorescence or fluorescent-related parameter generated.
Specification